Exporession vector for human TnF

Details Exporession vector for human TnF Exporession vector for human TnF

1986-09-16

1989-10-03

Teskin, Robin

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 4351723, 43525233, 435320, 536 27, 935 11, 935 29, 935 39, 935 42, 935 56, 935 65, C12P 2100, C12P 2102, C12N 1500, C12N 700

Patent

active

048716634

ABSTRACT:
The present invention provides a vector plasmid capable of efficient tumor necrosis factor (TNF) production, a process capable of efficient TNF production in a host transformed with said plasmid and a composition containing the TNF produced by said process.
The novel plasmid of the present invention is characterized by having inserted therein a DNA fragment that has a phage-derived promoter region upstream of a structural gene for TNF and in which a DNA fragment containing an E. coli gene-derived transcription termination coding base sequence (terminator) is joined immediately downstream of a base sequence coding for the termination of translation of said structural gene.

REFERENCES:
patent: 4677064 (1987-06-01), Mark et al.
Platt, Terry, Cell, vol. 24, pp. 10-23, Apr. 1981, "Termination of Transcription and Its Regulation in the Tryptophan Operum of E. coli. "
Lacatena et al., Cell, vol. 37, pp. 1009-1014, Jul. 1984, "Control of Initiation of pMB1 Replication: Purified Rop Protein and RNA I Affect Primer Formation In Vitro".
Nature, vol. 312, Dec. 20/27, 1984-D. Pennica et al, "Human Tumour Necrosis Factor: Precursor Structure, Expression and Homology to Lymphotoxin", pp. 724, 725.
Science, vol. 228, No. 4696, Apr. 12, 1985--A. M. Wang et al, "Molecular Cloning of the Complementary DNA for Human Tumor Necrosis Factor" pp. 149-154.

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