Expansion of bone marrow stromal cells

Details Expansion of bone marrow stromal cells Expansion of bone marrow stromal cells

1995-12-29

1998-06-16

Saunders, David

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Solid support and method of culturing cells on said solid...

435402, 435405, 435372, 530354, 530399, C12N 502, C12N 506, A61K 3818, A61K 3839

Patent

active

057669503

ABSTRACT:
The invention features a method for the selection and expansion of bone marrow stromal cells. The method includes the steps of obtaining bone marrow stromal cells; introducing the stromal cells into a vessel pre-coated on an inner surface with a gelatin, and containing a culture medium including an acidic fibroblast growth factor ("aFGF") polypeptide; and expanding the stromal cells in the culture medium under conditions and for a time sufficient to obtain an increased number of bone marrow stromal cells. The culture medium additionally can include heparin, and the vessel additionally can be precoated with fetal bovine serum.

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