Expansion of bone marrow stromal cells

Details Expansion of bone marrow stromal cells Expansion of bone marrow stromal cells

1998-06-15

1999-10-05

Saunders, David

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of culturing cells in suspension

435350, 435372, 435378, 435395, 435405, 530350, 536 21, C12N 506, C12N 508, C07K 1550, C08B 3710

Patent

active

059623233

ABSTRACT:
The invention features a method for the selection and expansion of bone marrow stromal cells. The method includes the steps of obtaining bone marrow stromal cells; introducing the stromal cells into a vessel pre-coated on an inner surface with a gelatin, and containing a culture medium including an acidic fibroblast growth factor ("aFGF") polypeptide; and expanding the stromal cells in the culture medium under conditions and for a time sufficient to obtain an increased number of bone marrow stromal cells. The culture medium additionally can include heparin, and the vessel additionally can be precoated with fetal bovine serum.

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