Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-02-22
2009-12-01
Wang, Andrew (Department: 1656)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S069100, C435S199000, C435S320100, C435S325000, C536S023200
Reexamination Certificate
active
07625717
ABSTRACT:
This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
REFERENCES:
patent: 6927042 (2005-08-01), Schultz et al.
patent: 7045337 (2006-05-01), Schultz et al.
patent: 7083970 (2006-08-01), Schultz et al.
patent: 7129333 (2006-10-01), Schultz et al.
patent: 7183082 (2007-02-01), Schultz et al.
patent: 7199222 (2007-04-01), Schultz et al.
patent: 7217809 (2007-05-01), Schultz et al.
patent: 7238510 (2007-07-01), Schultz et al.
patent: 7262040 (2007-08-01), Schultz et al.
patent: 2004/0198637 (2004-10-01), Schultz et al.
patent: 2004/0265952 (2004-12-01), Deiters et al.
patent: 2005/0009049 (2005-01-01), Chin et al.
patent: 2005/0181471 (2005-08-01), Schultz et al.
patent: 2005/0186656 (2005-08-01), Schultz et al.
patent: 2005/0208536 (2005-09-01), Schultz et al.
patent: 2005/0272121 (2005-12-01), Xie et al.
patent: 2006/0063244 (2006-03-01), Schultz et al.
patent: 2006/0068478 (2006-03-01), Schultz et al.
patent: 2006/0234367 (2006-10-01), Schultz et al.
patent: 2007/0172915 (2007-07-01), Schultz et al.
patent: 2007/0184517 (2007-08-01), Schultz et al.
patent: 2008/0227153 (2008-09-01), Schultz et al.
patent: 1 424 395 (2004-06-01), None
patent: WO 02/085923 (2002-10-01), None
patent: WO 02/086075 (2002-10-01), None
patent: WO 03/014354 (2003-02-01), None
Cropp et al. An expanding genetic code. Trends in Genetics vol. 20 No. 12 Dec. 2004, pp. 625-630.
Fukunaga et al Misacylation of Yeast Amber Suppressor tRNATyr byE. coliLysyl-tRNA Synthetase and Its Effective Repression by Genetic Engineering of the tRNA Sequenc. Journal of Biochemistry 2006 139(4):689-696.
Deiters et al. Adding amino acids with novel reactivity to the genetic code ofSaccharomyces cerevisiae. J Am Chem Soc. Oct. 1, 2003;125(39):11782-3.
Anderson et al., (2002)Exploring the Limits of Codon and Anticodon Size,Chemistry and Biology, 9:237-244.
Blake (2001)Cellular screening assays using fluorescence microscopy Curr. Opin. Pharmacol., 1:533-539.
Bolletta, F. et al., (1996)Synthesis and Photophysical Properties of Fluorescent Derivatives of Methylmercury, Organometallics15:2415-17.
Carboni, B et al., (1993)Aliphatic Amino Azides as Key Building Blocks for Efficient Polyamine Syntheses,J. Org. Chem. 58:3736-3741.
Chin et al., (2002),Addition of p-Azido-L-phenylalanine to the Genetic code of Escherichia coli,J. Am. Chem. Soc.124:9026-9027.
Chin and Schultz, (2002),In vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis,Chem BioChem11:1135-1137.
Chin, et al., (2002),Addition of a Photocrosslinker to the Genetic Code of Escherichia coli,Proc. Natl. Acad.Sci. U. S. A.99:11020-11024.
Chin, et al., (2003)Progress toward an expanded eukaryotic genetic code,Chem. Biol., 10(6):511-519.
Chin, et al., (2003)An expanded eukaryotic genetic code,Science, 301(5635):964-7.
Cornish, et al., (1996)Site-Specific Protein Modification Using a Ketone Handle,Journal of the American Chemical Society118:8150-8151.
Crisp, G. T.; & Gore, J. (1997)Preparation of Biological Labels with Acetylenic Linker Arms,Tetrahedron53:1505-1522.
Feng et al., (2003),Expanding tRNA recognition of a tRNA synthetase by a single amino acid change,PNAS100(10): 5676-5681.
Forster et al., (2003)Programming peptidomimetic synthetases by translating genetic codes designed de novo PNAS100(11):6353-6357.
Francklyn et al., (2002),Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation;RNA, 8:1363-1372.
Griffin, et al., (1998)Specific covalent labeling of recombinant protein molecules inside live cells,Science281:269-272.
Hamano-Takaku et al., (2000)A mutant Escherichia coli Tyrosyl-tRNA Synthetase Utilizes the Unnatural Amino Acid Azatyrosine More Efficiently than Tyrosine,Journal of Biological Chemistry, 275(51):40324-40328.
Kiga et al. (2002),An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukatyotic translation and its application in a wheat germ cell-free system,PNAS99(15): 9715-9723.
Kiick et al., (2002)Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligtation,PNAS99:19-24.
Lemineux, & Bertozzi, (1996)Chemoselective ligation reactions with proteins,oligosaccharides and cells,TIBTECH, 16:506-513.
Liu, D.R. & Schultz, P.G. (1999)Progress toward the evolution of an organism with an expanded genetic code.PNAS United States96:4780-4785.
Magliery, (2001)Expanding the Genetic Code: Selection of Efficient Suppressors of Four-base Codons and Identification of “Shifty” Four-base Codons with a Library Approach in Escherichia coli,J. Mol. Biol.307: 755-769.
Mahal, et al., (1997)Engineering chemical reactivity on cell surfaces through oligosaccharide biosynthesis,Science, 276:1125-1128.
Padwa, A. (1991)Intermolecular 1,3-Dipolar CycloaddtionsinComprehensive Organic Synthesis, vol. 4, Ed. Trost, B. M., Pergamon, Oxford, p. 1069-1109.
M. Pasternak, et al., (2000),A new orthogonal suppressor tRNA/aminoacyl-tRNA synthetase pair for evolving an organism with an expanded genetic code,Helvetica Chemica Acta83:2277.
Rostovtsev, et al., (2002)A stepwise Huisgen cycloaddition process: copper(l)-catalyzed regioselective “ligation” of azides and terminal alkynes,Angew. Chem. Int. Ed.41:2596-2599.
Speers, et al., (2003)Activity-based protein profiling in vivo using a copper(i)-catalyzed azide-alkyne[3+2]cycloaddition,J. Am. Chem. Soc., 125:4686-4687.
Tornøe, et al., (2002)Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(i)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides,Org. Chem.67:3057-3064.
Wang, et al., (2001),Expanding the genetic code of Escherichia coli,Science292:498-500.
Wang, et al., (2002),Adding L-3-(2-naphthyl)alanine to the genetic code of E-coli,J. Am. Chem. Soc.124:1836-1837.
Wang and Schultz, (2002),Expanding the Genetic Code,Chem. Comm.1-10.
Wang, et al., (2003)Addition of the keto functional group to the genetic code of Escherichia coli,Proc. Natl. Acad. Sci., 100:56-61.
Wang, et al., (2003)Bioconjugation by copper(l)-catalyzed azide-alkyne[3+2]cycloaddition.J. Am. Chem. Soc., 125:3192-3193.
Wouters, et al., (2001)Imaging biochemistry inside cells,Trends in Cell Biology11:203-211.
Zacharias, et al., (2000)Recent advances in technology for measuring and manipulating cell signals,Curr. Opin. Neurobiol., 10:416-421.
Zhang et al., (2002),The selective incorporation of alkenes into proteins in Escherichia coli,Angewandte Chemie. International Ed. in English41:2840-2842.
Zhang, et al., (2003)A new strategy for the site-specific modification of proteins in vivo,Biochemistry, 42:6735-6746.
Gallivan et al. (1997) “Site-Specific Incorporation of Biotinylated Amino Acids to Identify Surface Exposed Residures in Integral Membrane Priteins.”Chemistry and Biology, 4(10): 739-749.
Dougherty (2000) “Unnatural amino acids as probes of protein structure and function,”Current Opinions in Chemical Biology, 4:645-652.
Edwards & Schimmel (1990) “A bacterial amber suppressor insaccharomyces cerevisiaeis selectively recognized by a bacterial aminoacyl-tRNA synthetase,”Molecular and Cellular Biology, 10(4):1633-1641.
Eriani et al. (1990) “Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs.”Nature, 347: 203-206.
Kolb et al. (2001) “Click chemistry: diverse chemical function from a few good reactions,”Angew. Chem. Int. Ed., 40:2004-2021.
Lewis et al. (2002) “Click chemistry in situ: acetylcholinesterase as a reaction vessel for the selective assembly of a femtomolar inhibitor from an array of building blocks,”Angew. Chem. Int Ed., 41(6):1053-1057.
Anderson J. Christopher
Chin Jason W.
Cropp T. Ashton
Schultz Peter G.
Gebreyesus Kagnew H
Landry Stacy
Quine Intellectual Property Law Group
The Scripps Research Institute
Wang Andrew
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