Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1994-10-03
1998-04-21
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
435 41, 435176, 435177, 435180, 435181, 435183, 436524, 436528, 436531, 436532, 436547, 514 2, 530811, 530812, 530815, 530816, 530402, C12N 996, C07K 113, C08H 100
Patent
active
057416869
ABSTRACT:
A method is provided for preparing a labeled protein, immobilized protein or protein-bioactive agent composition by attaching a label, support or bioactive agent to a protein by exopeptidase catalysis at a site that is remote from the active site of the protein. More specifically, an amine or alcohol group of an amino acid, amine or alcohol nucleophile is reacted by exopeptidase catalysis with a C-terminus carboxylic acid group of a protein such as an antibody, enzyme or hormone to couple the nucleophile to the protein to form an adduct, and the adduct is bound to an auxiliary substance such as a support, label or bioactive agent or its combination with a linker arm by reacting a reactive substituent of the nucleophile with a reactive group of the auxiliary substance. Alternatively, the nucleophile is bound to the auxiliary substance or its combination with a linker arm to form an intermediate, and the intermediate is coupled by exopeptidase catalysis to the protein. The exopeptidase may be a serine or cysteine exocarboxypeptidase.
REFERENCES:
patent: 4889916 (1989-12-01), Packard et al.
patent: 4959306 (1990-09-01), Kameda et al.
patent: 5234820 (1993-08-01), Wagner et al.
Kauer, et al., The Journal of Biological Chemistry, vol. 261, No. 23, 1986, pp. 10695-10700.
Kauer, et al., J. Biol. Chem., 261, 10695-10700 (1986).
Koh, et al., Biotechniques, 7, 596-602 (1989).
Borman, S., Chemical and Engineering News, 25-28 (1989).
Dalton, et al., J. Biol. Chem., 103, 549-578 (1933).
Champion, et al., Immunology, 54, 513-519 (1985).
Luehr, et al., J. Biochem. Biophys. Methods, 3, 151-161 (1980).
Unnithan, et al., Anal. Biochem., 136, 195-201 (1984).
Knowles, et al., J. Biol. Chem., 247, 6617-6623 (1972).
Hayashi, et al., J. Biochem., 77, 69-79 (1975).
J.S. Fruton, "Proteinase-Catalyzed Synthesis of Peptide Bonds", Advances in Enzymology, 53, 239-306 (1982).
I. Roitt et al., "Antibody Structure", Immunology, C.V. Mosby Co., St. Louis, 5.3 (1985).
A. Good et al., "Purification of Immunoglobulins and Their Fragments", Selected Mejthods in Cellular Immunology, Mishell & Shugi, eds., W.H. Freeman & Co., San Francisco, 278-286 (1980).
K. Breddam, "Serine Carboxypeptidases. A. Review.", Carlsberg Res. Commun., 51, 83-128 (1986).
Coolidge Thomas R.
Lewis William
Schuster Sheldon M.
Stout Jay
Wagner Fred W.
Board of Regents of the University of Nebraska
Naff David M.
LandOfFree
Exopeptidase catalyzed site-specific bonding of supports, labels does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Exopeptidase catalyzed site-specific bonding of supports, labels, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Exopeptidase catalyzed site-specific bonding of supports, labels will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2056888