Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Patent
1990-06-28
1993-08-10
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
435 71, 435 792, 435181, 436532, 436544, 530816, C12P 100, G01N 33532, G01N 33547, C12N 1106
Patent
active
052348202
ABSTRACT:
An auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm is bound to the nucleophile to form an intermediate substance which is then coupled by catalysis with exopeptidase to the carboxy terminus of a protein.
REFERENCES:
Kauer, et al., The Journal of Biological Chemistry. vol. 261, No. 23, 1986, pp. 10695-10700.
Breddam Klaus
Coolidge Thomas R.
Lewis William
Schuster Sheldon M.
Stout Jay
BioNebraska, Inc.
Board of Regents of the University of Nebraska
Naff David M.
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