Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1989-06-23
1998-09-22
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 935 33, 935 41, C12N 1585, C12P 2102
Patent
active
058112600
ABSTRACT:
An expression vector for the expression of an exogenous gene in an animal cell, which contains a chick .beta.-actin gene promoter and a restriction enzyme site for incorporating an exogenous gene at the downstream of said promoter and a process for expressing an exogenous gene, which comprises incorporating said exogenous gene into the expression vector at the restriction enzyme site for incorporating the exogenous gene, introducing said vector into an animal cell and culturing the obtained transformed animal cell. The expression system of the present invention can be applied to an expression of any exogenous gene and has an extremely high expression efficiency and is applicable to a wide range of host cells, and hence can sufficiently be utilized for industrial-scale production of a useful material.
REFERENCES:
patent: 4740461 (1988-04-01), Kaufman
N. Fregien et al., "Activating Elements in the Promoter Region of the Chin .beta.-actin gene," Gene, vol. 48, 1986, pp. 1-11.
B. C. Guild et al., "Development of Retrovirus Vectors Useful for Expressing Genes in Cultured Murine Embryonal Cells and Hematopoietic Cells In Vivo," J. of Virology, Oct. 1988, pp. 3795-3801.
W. W. Quitschke et al., "The .beta.-Actin Promoter," J. of Biological Chemistry, vol. 264, No. 16, 1989, pp. 9539-9546.
Kost et al. Nucleic Acids Research, vol. 11, No. 23, 1983, pp. 8287-8300.
Araki Masatake
Miyazaki Jun-ichi
Nozaki Chikateru
Yamamura Ken-ichi
Yonemura Hiroshi
Juridical Foundation The Chemo-Sero Therapeutic Research Institu
Zitomer Stephanie W.
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