Exogenous gene expression vector containing chick .beta.-actin g

Details Exogenous gene expression vector containing chick .beta.-actin g Exogenous gene expression vector containing chick .beta.-actin g

1997-03-10

1998-06-23

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 4352402, 935 23, 935 27, C12N 1570, C12N 1585, C12P 2102

Patent

active

057704007

ABSTRACT:
An expression vector for the expression of an exogenous gene in an animal cell, which contains a chick .beta.-actin gene promoter and a restriction enzyme site for incorporating an exogenous gene at the downstream of said promoter and a process for expressing an exogenous gene, which comprises incorporating said exogenous gene into the expression vector at the restriction enzyme site for incorporating the exogenous gene, introducing said vector into an animal cell and culturing the obtained transformed animal cell. The expression system of the present invention can be applied to an expression of any exogenous gene and has an extremely high expression efficiency and is applicable to a wide range of host cells, and hence can sufficiently be utilized for industrial-scale production of a useful material.

REFERENCES:
patent: 4740461 (1988-04-01), Kaufman
Kost et al., Nucleic Acids Research, vol. 11(23):8287-8300, (1983).
Fregien et al., "Activating Elements In The Promoter Region Of The Chicken beta.-Actin Gene", Gene, vol. 48:1-11, (1986).
Guild et al., "Development of Retrovirus Vectors Useful For Expressing Genes In Cultured Murine Embryonal Cells And Hematopoietic Cells In Vivo", J. of Virology, pp. 3795-3801, (1988).
Quitschke et al., "The .beta.-Actin Promoter", J. of Biological Chemistry, vol. 264(16):9539-9546, (1989).

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