Chemistry: analytical and immunological testing – Metal or metal containing – Zn – cd – hg – sc – y – or actinides – or lanthanides
Reexamination Certificate
2003-09-30
2009-02-17
Soderquist, Arlen (Department: 1797)
Chemistry: analytical and immunological testing
Metal or metal containing
Zn, cd, hg, sc, y, or actinides, or lanthanides
C435S015000, C435S018000, C436S073000, C436S074000, C436S166000, C436S172000
Reexamination Certificate
active
07491545
ABSTRACT:
A highly selective and sensitive of a carbonic anhydrase-based method for measurement of zinc ion by an excitation ratiometric format based on resonance energy transfer: i.e., where the zinc ion level is transduced as the ratio of fluorescence intensities excited at two different excitation wavelengths, is provided. The method can be used very well in a fluorescence microscopy format. A detection limit of about 10 pM in zinc buffered systems, a ten to one thousand-fold improvement on the Fura indicators (which respond to Ca and Mg as well), and a one hundred thousand-fold improvement on the recently described FuraZin-1 is achieved.
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Bozym Rebecca
Cramer Michele
Fierke Carol Ann
Thompson Richard
Zeng Hui Hui
Birch & Stewart Kolasch & Birch, LLP
Soderquist Arlen
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