Surgery – Blood vessel or graft preparation
Reexamination Certificate
2006-03-14
2006-03-14
Lacyk, John P. (Department: 3736)
Surgery
Blood vessel or graft preparation
C623S916000
Reexamination Certificate
active
07011623
ABSTRACT:
The present invention provides an ex vivo vascular remodeling methods and system by which an excised, small diameter blood vessel can be harvested and expanded to provide viable vascular grafts, as demonstrated at the physical and molecular levels, and as optimized in vivo. The tissue-engineered vessels generated by the present invention closely resemble native vessels in terms of structure, histologically, including endothelial coverage and intricate structural components such as the internal elastic lamina, viability (as measured with the MTT assay and TUNEL analysis), and function (vasoactivity, mechanical and biomechanical properties). Thus, the resulting vascular grafts behave in a manner similar to native arteries in terms of mechanical integrity, and provide clinically relevant patency rates when implanted in vivo. Moreover, the ex vivo methods and system permit the precise control of the mechanical environment involving the excised vessel, while at the same time permitting careful monitoring of the resulting growth/remodeling, thereby opening new avenues of research regarding the mechanical stimuli responsible for specific aspects of remodeling in vivo.
REFERENCES:
patent: 6322553 (2001-11-01), Vito
Conklin, B.S., “Viability of Porcine Common Carotid Arteries i a Novel Organ Culture System,” MS Thesis, Georgia Institute of Technology, 1997.
Berceli, S., et al., “Biomechanics of the venous wall under simulated arterial conditions,”J. Biomech.23(10):985-989 (1990).
Bergel, D., “The dynamic elastic properties of the arterial wall,”J. Physiol.(Lond) 156:458-469 (1961).
Brant, A., et al., “Biomechanics of the arterial wall under simulated flow conditions,”J. Biomechanics21(2):107-113 (1988).
Campbell, J.H., et al., “Novel vascular graft grown within recipient's own peritoneal cavity,”Circ. Res.85(12):1173-1178 (1999).
Chesler, N.C., et al., “Transmural pressure induces matrix-degrading activity in porcine arteriesex vivo,”Am. J. Physiol.277(5 Pt 2):H2002-2009 (1999).
Chiquet-Ehrismann, R., et al., “The complexity in regulating the expression of tenascins,”Bioessays17(10):873-878 (1995).
Cho, et al., “Effects of changes in blood flow rate on cell death and cell proliferation in carotid arteries of immature rabbits,”Circ. Res.81(3):328-337 (1997).
Cowan, K.N., et al., “Regression of hypertrophied rat pulmonary arteries in organ culture is associated with suppression of proteolytic activity, inhibition of tenascin-C, and smooth muscle cell apoptosis,”Circ. Res.84(10):1223-1233 (1999).
Fitzgibbon, G.M., et al., “Coronary bypass graft fate and patient outcome: angiographic follow-up of 5,065 grafts related to survival and reoperation in 1,388 patients during 25 years,”J. Am. Coll. Cardiol.28(3):616-626 (1996).
Fung, Y.,Biomechanics: Circulation.2nd ed. (1996).
Fung, Y.C., et al., “Changes of zero-stress state of rat pulmonary arteries in hypoxic hypertension,”J. Appl. Physiol.70(6):2455-2470 (1991).
Gessener, U., et al., “Methods of determining the distensibility of blood vessels,”IEEE Trans. Biomed. Eng.13:2-10 (1966).
Gooch, K., et al.,Mechanical Forces: Their Effects on Cells and Tissues, Berlin:Springer, p. 182 (1997).
Gooch, K., et al., “Mechanical Forces Growth Factors in Tissue Engineering,” inFrontiers in Tissue Engineering, (C. Patrick, A. Mikos, and L. McIntire, Edit.) Pergamon, New York, p. 61-82 (1998).
Gooch, K.J., et al., “Flow- and bradykinin-induced nitric production by endothelial cells in independent of membrane potential,”Am. J. Physiol.270(2 Pt 1):C546-51 (1996).
Gooch, K.J., et al., “Exogenous, basal, and flow-induced nitric oxide production and endothelial cell proliferation,”J. Cell Physiol.171(3):252-258 (1997).
Herman, I.M., et al., “Hemodynamics and the vascular endothelial cytoskeleton,”J. Cell Biol.105(1):291-302 (1987).
Holman, E., “Problems in the dynamics of blood flow, I. Condition controlling collateral circulation in the presence of an arteriovenous fistula following the ligation of an artery,”Surgery26:889-917 (1949).
Jones, P.L., et al., “Tenascin-C is induced with progressive pulmonary vascular disease in rats and is functionally related to increased smooth muscle cell proliferation,”Circ. Res.79(6):1131-1142 (1996).
Jones, P.L., et al., “Induction of vascular smooth muscle cell tenascin-C gene expression by denatured type I collagen is dependent upon a beta3 integrin-mediated mitogen-activated protein kinase pathway and a 122-base pair promoter element,”J. Cell Sci.112(Pt 4):435-445 (1999).
Jones, P.L., et al., “Regulation of tenascin-C, a vascular smooth muscle cell survival factor that interacts with the alpha v beta 3 integrin to promote-epidermal growth factor receptor phosphorylation and growth,”J. Cell Biol.139(1):279-293 (1997).
Kamiya, A., et al., “Adaptive regulation of wall shear stress to flow change in the canine carotid artery,”Am. J. Physiol.239(1):H14-21 (1980).
Labadie, R.F., et al., “Pulsatile perfusion system for ex vivo investigation of biochemical pathways in intact vascular tissue,”Am. J. Physiol.270(2 Pt 2):H760-768 (1996).
L'Heureux, N., et al., “A completely biological tissue-engineered human blood vessel,”FASEB J.12(1):47-56 (1998).
L'Heureux, N., et al., “In vitro construction of a human blood vessel from cultured vascular cells: a morphologic study,”J. Vasc. Surg.17(3):499-509 (1993).
Liu, S.Q., “Biomechanical basis of vascular tissue engineering,”Crit. Rev. Biomed. Eng.27(1-2):75-148 (1999).
Loftus, I.M., et al., “MMP inhibition reduces intimal hyperplasia in a human vein graft stenosis model,”Ann. N Y Acad. Sci.878:547-550 (1999).
Lytle, B.W., et al., “Long-term (5 to 12 years) serial studies of internal mammary artery and saphenous vein coronary bypass grafts,”J. Thorac. Cardiovasc. Surg.89(2):248-258 (1985).
Mackie, E.J., “Molecules in focus: tenascin-C,”Int. J. Biochem. Cell Biol.29(10):1133-1137 (1997).
Masood, I., et al., “Endothelin-1 is a mediator of intimal hyperplasia in organ culture of human saphenous vein,”Brit. J. Surg.84(4):499-503 (1997).
Mavromatis, K., et al., “Early effects of arterial hemodynamic conditions on human saphenous veins perfused ex vivo,”Arterioscler. Thromb. Vasc. Biol.20(8): 1889-1895 (2000).
Melkumyants, A.M, et al, “Effect of blood viscocity on arterial flow induced dilator response,”Cardiovasc. Res.24(2):165-168 (1990).
Meng, X., K., et al., “Mechanical stretching of human saphenous vein grafts induces expression and activation of matrix-degrading enzymes associated with vascular tissue injury and repair,”Exp. Mol. Pathol.66(3):227-237 (1999).
Milnor, W.R.,Hemodynamics, 2nd ed., Baltimore, Williams & Wilkins, p. 419 (1989).
Mulvihill, D. and S. Harvey, “The mechanism of the development of collateral circulation,”N. Engl. J. Med.104:1032 (1931).
Niklason, L.E., et al., “Functional arteries grown in vitro,”Science284(5413):489-493 (1999).
Porter, K.E., et al., “Endothelin-B receptors mediate intimal hyperplasia in an organ culture of human saphenous vein,”J. Vasc. Surg.28(4):695-701 (1998).
Porter, K.E., et al., “Marimastat inhibits neointimal thickening in a model of human vein graft stenosis,”Brit. J. Surg.85(10):1373-1377 (1998).
Porter, K.E., et al., “Production and inhibition of the gelatinolytic matrix metalloproteinases in a human model of vein graft stenosis,”Eur J Vasc Endovasc Surg.17(5):404-412 (1999).
Porter, K.E., et al., “The development of an in vitro flow model of human saphenous vein graft intimal hyperplasia,”Cardiovasc. Res.31(4):607-614 (1996).
Sandusky, G.E., G.C. Lantz, and S.F. Badylak, “Healing comparison of small intesti
Clerin Valerie
Gooch Keith
Gusic Rebecca
Drinker Biddle & Reath LLP
Lacyk John P.
McConathy Evelyn H.
The Trustees of the University of Pennsylvania
LandOfFree
Ex vivo remodeling of excised blood vessels for vascular grafts does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Ex vivo remodeling of excised blood vessels for vascular grafts, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ex vivo remodeling of excised blood vessels for vascular grafts will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3529130