Combinatorial chemistry technology: method – library – apparatus – Method of screening a library – By measuring the ability to specifically bind a target...
Reexamination Certificate
2005-05-31
2008-10-28
Kim, Young J. (Department: 1637)
Combinatorial chemistry technology: method, library, apparatus
Method of screening a library
By measuring the ability to specifically bind a target...
C506S001000, C506S011000, C506S013000, C506S028000, C506S041000, C435S006120, C435S091200, C536S023100
Reexamination Certificate
active
07442160
ABSTRACT:
Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.
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Gartner Zev J.
Kanan Matthew W.
Liu David R.
Goodwin & Procter LLP
Kim Young J.
President and Fellows of Harvard College
Woolwine Samuel
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