Eukaryotic transposable element

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435 691, 435 701, 4351723, 4352402, 530350, 536 221, 536 231, 536 232, 536 235, 935 9, 935 19, C12N 916, C12N 500, C07H 1900, C07H 1700

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053488741

ABSTRACT:
Disclosed are isolated transposable elements, or isolated DNA sequences which encode a transposase protein (or a portion of a transposase protein). The isolated transposable elements or the isolated DNA sequences being characterized by the ability to hybridize to the DNA sequence of Minos 1 under stringent hybridization conditions. The invention also relates to a purified transposase protein, or peptide fragments thereof, encoded by such DNA sequences. Such transposable are useful in methods for the stable introduction of a DNA sequence of interest into a eukaryotic cell. The sequence information disclosed herein is useful in the design of oligonucleotide primers which are useful for the isolation of related members of the Tc-1 family of transposable elements.

REFERENCES:
Minos-2 DNA Sequence submitted to EMBL Data Library by Charalambos Savakis; Released by EMBL Data Library on Sep. 12, 1991.
Franz and Savakis, Nucl. Acids Res. 19(23): 6646, Dec. 11, 1991.

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