Eukaryotic transposable element

Details Eukaryotic transposable element Eukaryotic transposable element

1992-09-14

1994-09-20

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 435 701, 4351723, 4352402, 530350, 536 221, 536 231, 536 232, 536 235, 935 9, 935 19, C12N 916, C12N 500, C07H 1900, C07H 1700

Patent

active

053488741

ABSTRACT:
Disclosed are isolated transposable elements, or isolated DNA sequences which encode a transposase protein (or a portion of a transposase protein). The isolated transposable elements or the isolated DNA sequences being characterized by the ability to hybridize to the DNA sequence of Minos 1 under stringent hybridization conditions. The invention also relates to a purified transposase protein, or peptide fragments thereof, encoded by such DNA sequences. Such transposable are useful in methods for the stable introduction of a DNA sequence of interest into a eukaryotic cell. The sequence information disclosed herein is useful in the design of oligonucleotide primers which are useful for the isolation of related members of the Tc-1 family of transposable elements.

REFERENCES:
Minos-2 DNA Sequence submitted to EMBL Data Library by Charalambos Savakis; Released by EMBL Data Library on Sep. 12, 1991.
Franz and Savakis, Nucl. Acids Res. 19(23): 6646, Dec. 11, 1991.

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