Eukaryotic signal sequences for prokaryotic expression

Details Eukaryotic signal sequences for prokaryotic expression Eukaryotic signal sequences for prokaryotic expression

2006-08-22

2006-08-22

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

Reexamination Certificate

active

07094579

ABSTRACT:
The present invention generally relates to methods and compositions for expressing proteins or polypeptides in prokaryotic hosts using eukaryotic signal sequences.

REFERENCES:
patent: WO 98/47343 (1998-10-01), None
patent: WO 00/47741 (2000-08-01), None
No references cited.
Supplementary European Search Report PCT/US03/04791, Dec. 20, 2005 (5 total pages).
Piers, K.L., Brown, M.H., Hancock, R.E.W.,Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria, GENE 134 (1992) 7-13.
Haldimann, A., Daniels, L.L., Wanner, B.L.,Use of New Methods for Construction of Tightly Regulated Arabinose and Rhamnose Promoter Fusions in Studies of the Escherichia coli Phosphate Regulon, Journal of Bacteriology, Mar. 1998, pp. 1277-1286.
Humphreys, D.P., Sehdev, M., Chapman, A.P., Ganesh, R., Smith, B.J., King, L.M., Glover, D.J., Reeks, D.G., Stephens, P.E.,High-Level Periplasmic Expression in Escherichia coli Using a Eukaryotic Signal Peptide: Importance of Codon Usage at the 5′ End of the Coding Sequence, Protein Expression and Purification 20, pp. 252-264 (2000).

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