Eukaryotic signal sequences for polypeptide expression and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S007100, C435S320100, C530S387100

Reexamination Certificate

active

07396661

ABSTRACT:
The present invention generally relates to methods and compositions for expressing proteins or polypeptides in prokaryotic hosts using eukaryotic signal sequences.

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patent: WO 00/47741 (2000-08-01), None
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Strobel et al in “Efficient Display of Two Enzymes on Filamentous Phage Using an Improved Signal Sequence” in Molecular Biotechnology, vol. 24, (2003) pp. 1-9.
Luis Angel Fernández, “Prokaryotic expression of antibodies and affibodies” in Current Opinion in Biotechnology,Aug. 2004, pp. 364-373.
Supplementary European Search Report PCT/US03/04791, Dec. 20, 2005 (5 total pages).
Piers, K.L., Brown, M.H., Hancock, R.E.W.,Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria, Gene 134 (1992) 7-13.
Haldimann, A., Daniels, L.L., Wanner, B.L.,Use of New Methods for Construction of Tightly Regulated Arabinose and Rhamnose Promoter Fusions in Studies of the Escherichia coli Phosphate Regulon, Journal of Bacteriology, Mar. 1998, pp. 1277-1286.
Humphreys, D.P., Sehdev, M., Chapman, A.P., Ganesh, R., Smith, B.J., King, L.M., Glover, D.J., Reeks, D.G., Stephens, P.E.,High-Level Periplasmic Expression in Escherichia coli Using a Eukaryotic Signal Peptide: Importance of Codon Usage at the 5′ End of the Coding Sequence, Protein Expression and Purification 20, pp. 252-264 (2000).
Supplementary Partial European Search Report; Application No. 03739833.6-2406; PCT/US0304791, Dec. 20, 2005.

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