Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1985-07-29
1989-10-24
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
43 68, 43 7C, 43255, 43256, 433171, 43320, 536 27, 935 28, 935 37, 935 41, 935 69, C12P 1500, C12P 2100, C12P 21, C12N 700
Patent
active
048761974
ABSTRACT:
Methods and compositions are provided for efficient expression of genes in unicellular microorganisms, particularly yeast. The systems involve an expression system employing transcriptional initiation regions from glycolytic enzymes, particularly a chimeric expression system, having a first region providing for regulatable or constitutive expression, a second region providing for transcriptional initiation, where regions one and two are not found joined together in functional relationship in nature, and optionally a sequence providing for a secretory leader and processing signal, where the expression cassette will be joined to a gene which may be homologous or heterologous to the host. The expression cassette can be used on an extrachromosomal element or integrated into the host genome, whereby continuous expression can be achieved or inducible expression is obtained, by virtue of the presence or absence of an inducer. Constructions may be provided, where structural genes may be introduced in reading frame with the cassette to provide for expression of the natrual peptide, a fused peptide, or a peptide precursor.
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Burke Rae Lyn
Rosenberg Steven
Shuster Jeffrey R.
Tekamp-Olson Patricia A.
Valenzuela Pablo D. T.
Chiron Corporation
Seidman Stephanie
Wiseman Thomas G.
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