Chemistry: molecular biology and microbiology – Vector – per se
Patent
1996-01-23
1999-03-23
Ketter, James
Chemistry: molecular biology and microbiology
Vector, per se
4353722, C12N 1500
Patent
active
058858270
ABSTRACT:
A method is provided for performing saturation mutagenesis on a target gene by exploiting the immunoglobulin hypermutation system. A target gene is cloned into an expression vector containing immunoglobulin enhancer fragments that effect hypermutation, and this construct is then transfected into an immunoglobulin mutator cell, typically of pre-B lymphocyte lineage. The target gene is permitted to hypermutate at a rate approaching that of 10.sup.-4 /bp/generation as the cells are cultured to a desired density. The variant polypeptides encoded by the hypermutated target gene can then be selected.
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Bachl Jurgen
Wabl Matthias
Ketter James
The Regents of the Universtiy of California
Yucel Irem
LandOfFree
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