Eukaryotic expression vectors with multimeric enhancer segments,

Details Eukaryotic expression vectors with multimeric enhancer segments, Eukaryotic expression vectors with multimeric enhancer segments,

1988-06-29

1991-01-08

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435320, C12N 1567, C12N 1585

Patent

active

049835181

ABSTRACT:
The present invention provides a eukaryotic expression vector containing a gene to be expressed and a TATA box, whereby, 1-200 bp base pair (bp) upstream (5') of the TATA box, there is a first multimeric enhancer segment and 1-100 bp below (3') the gene to be expressed there is provided a second multimeric enhancer segment. The present invention also provides a process for the preparation of this expression vector. Furthermore, the present invention is concerned with the use of this expression vector.

REFERENCES:
Zenke, M. et al., Embo J., 5(2), 387-397 (1986).
Searle et al., (1985) Mol Cell Biol, 5:1480-1489.
Kumar et al., (1986) PNAS, 83: 3199-3203.
Veldman et al., (1985) Mol Cell Biol, 5:649-658.
Toohey et al., (1986) Mol Cell Biol, 6:4526-4538.
Mann et al., (1983) Cell 33: 153-159.
Miller et al., (1984) Science, 225:993-998.

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