Eukaryotic cells stably expressing genes from multiple transfect

Details Eukaryotic cells stably expressing genes from multiple transfect Eukaryotic cells stably expressing genes from multiple transfect

1998-08-06

1999-11-02

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435369, 435467, C12Q 168

Patent

active

059768070

ABSTRACT:
A method is described for producing recombinant eukaryotic cell lines expressing multiple proteins of interest. Eukaryotic host cells are transfected with (a) a first episome which contains an EBV origin of replication and a first gene encoding a protein of interest; and (b) a second episome containing an EBV origin of replication and a second gene encoding a protein of interest. Transfected cells are obtained expressing an EBNA 1 protein. The cells are grown under conditions wherein the episomes express the first and second genes.

REFERENCES:
Horlick et al., Prot. Expr. and Purif., vol. 9, 1977, pp. 301-308, 1997.

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