Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1997-05-09
1999-07-06
Degen, Nancy
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 912, 4353201, 435325, 435375, 536 241, 536 245, 536 253, C07H 2104, C12N 516, C12N 1511, C12P 1934
Patent
active
059196777
ABSTRACT:
Identified are genetic regulatory elements which are part of a natural antisense RNA negative regulatory system in eukaryotic cells. The genetic regulatory elements, designated an antisense initiator sequence, when downstream of and operably linked to a DNA molecule can transcribe the DNA molecule into RNA transcripts of negative strand polarity that function to bind to, in forming an RNA duplex with, sense RNA transcripts being produced from a target gene to be regulated. The invention relates to recombinant vectors useful for introduction into eukaryotic cells, and methods of using the vectors to regulate expression of a target gene comprising introducing the recombinant vectors into host eukaryotic cells.
REFERENCES:
Gielow et al. Expression of the replication protein Arp of phasyl shows dual regulation by an antisense promoter. EMBO J. 10(10): 3061-3066, 1991.
Lefranc et al. gd lineage specific transcription of human T cell receptor g genes by a combination of a non-lineage-specfic enhancer and silencers. Eur. Immunol. 25: 617-622, 1995.
Malik et al. Identification of an antisense WT1 promoter in intron 1: implications for WT1 gene regulation. Oncogene 11: 1589-1595, 1995.
Spicer et al. An antisense promoter of the murine c-myc gene is localized within intron 2. Mol. Cell. Biol. 12(3): 1324-1329, Mar. 1992.
Sawada et al. A lineage-specific transcriptional silencer regulates CD4 gene expression during T lymphocyte development. Cell 77: 917-929, Jun. 1994.
Winoto et al. ab lineage specific expression of the a T cell receptor gene by nearby silencers. Cell 59: 649-655, Nov. 1989.
Michael et al., "Negative Strand RNA Transcripts are Produced in HIV-1-Infected Cells and Patients by a Novel Promoter Downregulated by Tat", J. Virology, vol. 68, pp. 979-987, 1994.
Miller, "Human Immunodeficiency Virus May Encode a Novel Protein on Genomic DNA Plus Strand", Science, vol. 239, pp. 1420-1422, 1988.
Degen Nancy
Larson Thomas G.
The Research Foundation of State University of New York
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