Eukaryotes expressing single stranded hybrid molecules

Details Eukaryotes expressing single stranded hybrid molecules Eukaryotes expressing single stranded hybrid molecules

1995-07-25

1998-12-15

Ketter, James

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352404, 43525421, 4353201, 536 252, C12N 510, C12N 115

Patent

active

058495630

ABSTRACT:
Eucaryotic cells like mammal and plant cells transfected with genes, called retrons, which code for stable single-stranded hybrid molecules (msDNA) containing RNA and DNA portions are disclosed. The retrons producing said linked RNA and DNA portions of the msDNAs also contain a gene encoding a reverse transcriptase (RT), which is necessary for the synthesis of the msDNAs. The msDNAs may contain foreign nucleic acid fragments which may be antisense fragments. These msDNA antisense vectors are useful for targeting genes of target proteins.

REFERENCES:
patent: 5405775 (1995-04-01), Inouye et al.
patent: 5436141 (1995-07-01), Miyata et al.

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