Ethanol production using engineered mutant E. coli

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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435189, 435190, 43525233, 4351721, 4351723, 935 60, 935 73, C12P 706, C12N 120, C12N 902

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050285394

ABSTRACT:
The subject invention concerns novel means and materials for producing ethanol as a fermentation product. Mutant E. coli are transformed with a gene coding for pyruvate decarboxylase activity. The resulting system is capable of producing relatively large amounts of ethanol from a variety of biomass sources.

REFERENCES:
Fried, V. A., A. Novick (1973) "Organic Solvents as Probes for the Structure and Function of the Bacterial Membrane: Effects of Ethanol on the Wild Type and an Ethanol-Resistant Mutant of Escherichia coli K-12," J. Bacteriol. 114(1):239-248.
Ingram, L. O. (1976) "Adaptation of Membrane Lipids to Alcohols," J. Bacteriol. 125(2):670-678.
Ingram, L. O., N. S. Vreeland (1980) "Differential Effects of Ethanol and Hexanol on the Escherichia coli Cell Envelope," J. Bacteriol. 144(2):481-488.
Ingram, L. O., T. M. Buttke (1984) "Effects of Alcohols on Micro-Organisms," In Advances in Microbial Pathology, vol. 25, Academic Press, London; pp. 254-295.
Ingram, L. O. T. Conway, D. P. Clark, G. W. Sewell, and J. F. Preston (1987) "Genetic Engineering of Ethanol Production in Escherichia coli," Appl. Env. Microbiol. 53(10):2420-2425.
Conway, T., Y. Osman, J. I. Konna, E. M. Hoffmann, and L. O. Ingram (1987) "Promoter and Nucleotide Sequences of the Zymomonas mobilis Pyruvate Decarboxylase," J. Bacteriol. 169(3):949-954.
Brau, B. and H. Sahm (1986) "Cloning and Expression of the Structural Gene for Pyruvate Decarboxylase of Zymomonas mobilis in Escherichia coli," Arch. Microbiol. 144:296-301.
Clark et al., J. Mol. Evol., vol. 25(2); pp. 151-8 (1987).
Conway et al., J. Bacteriology, vol. 169(3); pp. 949-954 (1987).

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