Ethanol production in recombinant hosts

Details Ethanol production in recombinant hosts Ethanol production in recombinant hosts

2005-02-01

2005-02-01

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S162000, C435S163000, C435S165000, C435S252300, C435S252310, C435S320100

Reexamination Certificate

active

06849434

ABSTRACT:
The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable fromZymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.

REFERENCES:
patent: 4493893 (1985-01-01), Mielenz et al.
patent: 4612287 (1986-09-01), Coleman et al.
patent: 4839286 (1989-06-01), Backman
patent: 5482846 (1996-01-01), Ingram et al.
patent: 5916787 (1999-06-01), Ingram et al.
Barbosa, et al.,Current Microbiol. 28:279-282 (1994).
Beall, D.S. et al. (1993) “Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose”J. Ind. Microbiol.l 11:151-55.
Breau, B. et al. (1986) “Cloning and expression of the structural gene for pyruvate decarboxylase ofZymomonas mobilisinEscherichia coli” Arch. Microbiol. 144:296-301.
Bringer-Meyer, S. et al. (1986) “Pyruvate decarboxylase fromZymomonas mobilis. Isolation and partial characterization”Arch. Microbiol. 146:105-110.
Brock, et al.,Biology of Microorganisms, 4thEdition, Prentice-Hall, Inc., Englewood Cliffs, N.J., 1984, pp. 803-805.
Coleman, et al., J. Bacteriol. 169:4302-4307 (1987).
Conway T, et al. Cloning and Expression of Ethanologenic and Glycolytic Genes formZymomonas mobilisinE. coliAbstracts. Of the Annual Meeting of the American Society for Microbiology 1987: pp. 159.
Conway, T. et al. (1987) “Cloning and sequencing of the Alcohol Dehydrogenase II gene fromZymomonas mobilis”Journal of Bacteriology169(6):2591-7.
Danilevich, et al.,Molecular Biology28:158-166 (1994).
Gold, et al.,J. Ind. Microbiol. 10:45-54 (1992).
Gong, C-S. et al. (1981) “Production of ehtanol from D-Xylose by using D-Xylose isomerase and yeasts”Applied and Environmental Microbiology41(2):430-6.
Hashiba, et al.,Biosci. Biotech. Biochem. 56:190-194 (1992).
Ingram, L.O. et al. (1987) “Genetic Engineering of ethanol production inEscherichia coli” Applied and Environmental Microbiology53(10):2420-25.
Ingram, L.O. et al. (1988) “Expression of different levels of ehtanologenic enzymes fromZymomonas mobilisin recombinant strains ofEschirichia coli” Applied and Environmental Microbiology54(2):397-404.
Ingram. L.O. et al., “Genetic Engineering of Ethanol Production inEscherichia coli,” Appl. Environ. Microbiol. 53(10):2420-2425 (Oct. 1987).
Koide, Y. et al. (1986) “Cloning and sequencing of the major intracellular serine protease gene ofBacillus subtilis” Journal of Bacteriology167(1):110-6.
Lawford, et al.,Appl. Biochem. Biotechnol. 28/29:221-236 (1991).
Mistry, F.R. et al. (1989) “Production of ethanol byClostridium thermosaccharolyticum: I. Effect of cell recycle and environmental parameters”Biotechnology and Bioengineering34:1295-304.
Mistry, F.R. et al. (1989) “Production of ethanol byClostridium thermosaccharolyticum: II. A quantitative model describing product distributions”Biotechnology and Bioengineering34:1305-20.
Neale, A.D. et al. (1987) “Nucleotide sequence of the pyruvate decarboxylase gene fromZymomonas mobilis” Nucleic Acids Research15(4):1753-61.
O'Hara, M.B. et al. (1990) “Energy and calcium ion dependence of proteolysis during sporulation ofBacillus subtiliscells”Journal of Bacteriology172(8):4161-70.
Panbangred, et al.,Appl. Microbiol. Biotechnol. 22:259-264 (1985).
Sarthy, A.V. et al. (1987) “Expression of theEscherichia colixylose isomerase gene inSaccharomyces cerevisiae” Applied and Environmental Microbiology53(9):1996-2000.
Sneath et al., Eds., Bergey's Manual of Systematic Biology, vol. 2, Williams & Wilkins, Baltimore, MD, 1986, pp. xxi-xxiii.
Tolan, J.S. et al. (1987) “Fermentation of D-Xylose and L-Arabinose to ethanol byErwinia chrysanthemi” Applied and Environmental Microbiology53(9):2033-8.
Tolan, J.S. et al. (1987) “Fermentation of D-Xylose to ethanol by genetically modifiedKlebsiella planticola” Applied and environmental microbiology 53(9):2039-44.
Wood, B.E. (1992) “Ethanol production from cellobiose, amorphous cellulose, and crystalling cellulose by recombinantKlebsiella oxytocacontaining chromosomally integratedZymomonas mobilisgenes for ethanol production and plasmids expressing thermostable cellulase genes fromClostridium thermocellum” Applied and environmental microbiology58(7):2103-10.
Alterthum, F. et al. “Efficient ethanol production from glucose, lactose, and xylose by recombinantEscherichia coli.” Applied And Enviromental Microbiology55(8):1943-8 (1989).
Al-Zaag, A. “Molecular cloning of cellobiose and other β-glucosidase determinants fromKlebsiella oxytoca,” J. Biotechnol. 12:79-86 (1989).
Conway, T. et al., “Expression vector forZymomonas mobilis,” Appl. Environ. Microbiol. 53(2):235-241 (Feb. 1987).
Cornet et al. “Characterization of two cel (cellulose degradation) genes ofClostridium thermocellumcoding for endoglucanases”Bio/Technology1:589-594 (Sep. 1983).
Curry, C. et al. “Expression and Secretion of aCellulomonas fimiExoglucanase inSaccharomyces cerevisiae”, Applied And Environmental Microbiology, 54(2):476-484 (1988).
Grépinet, O. et al. “Purification ofClostridium thermocellumxylanase Z expressed inEscherichia coliand identification of the corresponding product in the culture medium ofC. thermocellum.” J Bacteriol. 170(10):4576-81 (1988).
Grépinet, O. et al. “Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) ofClostridium thermocellum,” J. Bacteriol. 170(10):4582-4588 (Oct. 1988).
Joliff, G. et al., “Isolation, crystallization and properties of a new cellulase ofClostridium thermocellumoverproduced inEscherichia coli,” Bio/Technology4:896-890 (Oct. 1986).
Kotoujansky, A, et al. “Molecular cloning ofErwinia chrysanthemipectinase and cellulase structural genes”The EMBO Journal4(3):781-785 (1985).
Millet, J. et al. “Cloning of ten distinct DNA fragments ofClostridium thermocellumcoding for cellulases,”FEMS Microbiol. Lett. 29:145-149 (1985).
Pétré, D. et al. “Purification and Properties of the endoglucanase C ofClostridium thermocellumproduced inEschericia coli,” Biochimie68:687-695 (1986).
Tailliez, P. et al. “Cellulose fermentation by an asporogenous mutant and an ethanol-tolerant mutant ofClostridium thermocellum,” Appl. Environ. Microbiol. 55(1):203-206 (Jan. 1989).

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