Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1993-03-05
1996-09-10
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435162, 4351723, C12P 710, C12P 714
Patent
active
055545203
ABSTRACT:
Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.
REFERENCES:
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Ohta, K. et al.; Applied Environ. Microbiol. 57:2810-2815 (1991).
Wong, W. K. R. et al.; Bio/Technology 6:713-719 (1988).
Esser, K. et al.; Proc. Biochem. 17(3):46-49 (1982).
Lawford, H. G. et al.; Biotechnol. Lett. 13:191-196 (1991).
Grepinet, O. et al.; J. Bacteriol. 170:4576-4581 (1988).
Mes-Hartree, M. et al.; Biotechnol. Bioeng. 30:558-564 (1987).
Ben-Bassat Arie
Fowler David E.
Horton Philip G.
Bioenergy International, L.C.
Grimes Eric
Wax Robert A.
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