Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1992-03-06
1995-06-13
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435165, 4352523, 4353201, 435847, 435854, C12N 121, C12N 1563, C12P 706, C12P 712
Patent
active
054242029
ABSTRACT:
Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.
REFERENCES:
patent: 4493893 (1985-01-01), Mielenz et al.
patent: 4612287 (1986-09-01), Coleman et al.
patent: 4839286 (1989-06-01), Backman
patent: 5000000 (1991-03-01), Ingram et al.
Raven, P. H. et al.; Biology of Plants, Worth Publishers, Inc., New York, 1981, pp. 251-255.
Herbert, R. A.; Trends Biotechnol. 10:395-402 (1992).
Brau, B. et al.; Arch. Microbiol. 144:296-301 (1986).
Al-Zaag, A: Molecular Cloning or Cellobiose and Other .beta.-Glucosidase Determinants from Klebsiella Oxytoca. J. Biotechnol. (1989) 12:79-86.
Alterthum, F. and Ingram, L. O.: Efficient Ethanol Production from Glucose, Lactose, and Xylose by Recombinant Escherichia coli. App. Env. Microbiol. (1989) 55:1943-1948.
Conway, T. et al.: Cloning and Expression of Ethanologenic and Glycolytic Genes from Zymomonas mobilis in E. coli. Abstr. Annu. Meet. Am. Soc. Microbiol. 1987, p. 159.
Gilkes, N. R. et al.: A Mutant of Escherichia coli that Leaks Cellulase Activity Encoded by Cloned Cellulose Genes from Cellulomonas fimi. Bio/Technol. (1984) 2:259-263.
Ohta, K. et al.: Metabolic Engineering of Klebsiella Oxytoca M5A1 for Ethanol Production from Xylose and Glucose. App. Env. Microbiol. (1991) 57:2810-2815.
Tolan, J. S. and Finn, R. K.: Fermentation of D-Xylose and L-Arabinose to Ethanol by Erwinia chrysantemi. App. Env. Microbiol. (1987) 53:2033-2038.
Hall, B. G. and Faunce, W.: Functional Genes for Cellulose Utilization in Natural Isolates of Escherichia coli. J. Bacteriol. (1987) 169:2713-2717.
Grepinet, O. et al.: Purification of Clostridium Thermocellum Xylanase 2 Expressed in Escherichia coli and Identification of the Corresponding Product in the Culture Medium of C. thermocellum. J. Bacteriol. (1988) 170:4576-4581.
Ingram, L. O. et al.: Genetic Engineering of Ethanol Production in Escherichia coli. App. Env. Microbiol. (1987) 53:2420-2425.
Kotoujansky, A. et al: Molecular Cloning of Erwinia Chrysanthemi Pectinase and Cellulase Structural Genes, Embo J. (1985) 4:781-785.
Millet, J. et al.: Cloning of Ten Distinct DNA Fragments of Clostridium thermocellum Coding for Cellulases. Fems Microbiol. Lett. (1985) 29:145-149.
Neale et al, "Nucleotide Sequence of the Pyruvate Decarboxylase Gene from Zymomonas mobilis", Nucleic Acids Research, vol. 15, No. 4, 1987, pp. 1753-1762.
Brau et al, "Cloning and Expression of the Structural Gene for Pyruvate Decarboxylase of Zymomonas mobilis in Escherichia coli", Arch Microbiol, 1986, 144:296-301.
Conway et al, "Cloning and Sequencing of the Alcohol Dehydrogenase II Gene from Zymomonas mobilis", Journal of Bacteriology, Jun. 1987, vol. 169, No. 6, pp. 2591-2597.
Curry et al, "Expression and Secretion of a Cellulomonas fimi Exoglucanase in Saccharomyces cerevisiae", Appl. Environ. Microbiol., Feb. 1988, vol. 54, No. 2, pp. 476-484.
Conway et al., "Expression Vector for Zymomonas mobilis", Appl. Environ. Microbiol., Feb. 1987, vol. 53, No. 2, pp. 235-241.
Tolan et al., "Fermentation of D-Xylos to Ethanol by Genetically Modified Klebsielle planticola", Appl. Environ. Microbiol., Sep. 1987, vol. 53, No. 9, pp. 2039-2044.
Joliff et al., "Isolation, Crystallization and Properties of a New Cellulase of Clostridium Thermocellum Overproduced in Escherichia coli" Bio/Technology, vol. 4, Oct. 1986, pp. 896-890.
Comet et al., "Characterization of Two Cel(Cellulose Degradation) Genes of Clostridium Thermocellum Coding for Endoglucanases", Bio/Technology, Sep. 1983, pp. 589-594.
Tailliez et al., "Cellulose Fermentation by an Asporogenous Mutant and an Ethanol-Tolerant Mutant of Clostridium thermolocellum", Appl. Environ. Microbiol., vol. 55, No. 1, Jan. 1989, pp. 203-206.
Petre et al., "Purification asnd Properties of the Endoglucanase C of Clostridium thermocellum Produced in Escherichia coli", Biochimie, vol. 68, 1986, pp. 687-695.
Grepinet et al., "Nucleotide Sequence and Deletion Analysis of the Xylanase Gene (xynZ) of Clostridium thermocellum", J. Bacteriol., vol. 170, No. 10, Oct. 1988, pp. 4582-4588.
Beall David S.
Burchhardt Gerhard F. H.
Guimaraes Walter V.
Ingram Lonnie O.
Ohta Kazuyoshi
Grimes Eric
Patterson Jr. Charles L.
The University of Florida
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