Ethanol production by recombinant hosts

Details Ethanol production by recombinant hosts Ethanol production by recombinant hosts

1992-09-17

1996-01-30

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435209, 4352523, 435852, 435162, C12N 121, C12N 942, C12P 710, C12P 714

Patent

active

054879892

ABSTRACT:
Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.

REFERENCES:
patent: 4321328 (1982-03-01), Hoge
Al-Zaag, J. Biotechnol. 12:79-86 (1989).
Mes-Hartree et al., Can. Bioenergy R. & D. Seminar (Proc.), 5th, 1984, pp. 469-472, Elsevier Appl. Sci, London.
Ohta et al., Appl. Env. Microbiol. 57:2810-2815 (1991).
Grepinet et al., J. Bacteriol. 170:4576-4581 (1988).
Wong et al., Bio/Technology 6:713-719 (1988).
Biely, Trends Biotechnol. 3:286-290 (1985).
Wood et al., Appl. Env. Microbiol. 58:12103-2110 (1992).

Affiliated with

Also associated with

Rating

Ethanol production by recombinant hosts does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ethanol production by recombinant hosts, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ethanol production by recombinant hosts will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-156332