ESTs and encoded human proteins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S325000, C435S252300, C435S254200, C435S320100, C536S023400

Reexamination Certificate

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07413875

ABSTRACT:
The sequences of 5′ ESTs and consensus contigated 5′ESTs derived from mRNAs encoding secreted proteins are disclosed. The 5′ ESTs and consensus contigated 5′ESTs may be to obtain cDNAs and genomic DNAs corresponding to the 5′ ESTs and consensus contigated 5′ESTs. The 5′ ESTs and consensus contigated 5′ESTs may also be used in diagnostic, forensic, gene therapy, and chromosome mapping procedures. Upstream regulatory sequences may also be obtained using the 5′ ESTs and consensus contigated 5′ESTs. The 5′ ESTs and consensus contigated 5′ESTs may also be used to design expression vectors and secretion vectors.

REFERENCES:
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patent: WO99/47540 (1999-09-01), None
patent: WO99/53051 (1999-10-01), None
GenBank® Accession No. AI138881 (Oct. 28, 1998).
Adams, M.D., et al. “3,400 New Expressed Sequence Tags Identify Diversity of transcripts in Human Brain,”Nature Genetics(Jul. 1993), vol. 4, pp. 256-267.
Adams, M.D., et al. “Rapid CDNA Sequencing (Expressed Sequenced Tags) from a Directionally Cloned Human Infant Brain CDNA Library,”Nature Genetics(Aug. 1993), vol. 4, pp. 373-380.
Tashiro, Kei, et al. “Signal Sequence Trap: A Cloning Strategy for Secreted Proteins and Type 1 Membrane Proteins,”Science(Jul. 30, 1993), vol. 261, pp. 600-603.
Carninci, Piero, et al. “High-Efficiency Full-Length CDNA Cloning by Biotinylated Cap Trapper”,Genomics(Nov. 1, 1996), vol. 37, pp. 327-336.
Kato, Seisho, et al. “Construction of a Human Full-Length CDNA Bank,”Gene(Dec. 15, 1994), vol. 150, pp. 243-250.

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