Esterase genes, esterase, recombinant plasmids and transformants

Details Esterase genes, esterase, recombinant plasmids and transformants Esterase genes, esterase, recombinant plasmids and transformants

1992-05-13

1994-05-03

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435196, 4353201, 536 232, C12A 1500

Patent

active

053087658

ABSTRACT:
The present invention relates to a DNA fragment containing a nucleotide sequence that encodes an amino acid sequence of esterase, said esterase asymmetrically hydrolyzing carboxylic acid esters represented by the formula (I); ##STR1## (wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 are alkyl, and n is 1 or 2) an esterase encoded by the DNA fragment, a recombinant plasmid containing the DNA fragment, a microorganism transformed with the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters.

REFERENCES:
Choi et al., 1990, Agric. Biol. Chem. 54(8):2039-2045.
Kamphuis et al., 1987, Proc. 4th Eur. Congr. Biotechnol., 4:331-348.
London et al., 1984, App. Environ. Microbiol., 47(5):993-997.
European Search Report dated Sep. 30, 1992-EP 92 10 8205.

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