Esterase genes, esterase, recombinant plasmids and transformants

Details Esterase genes, esterase, recombinant plasmids and transformants Esterase genes, esterase, recombinant plasmids and transformants

1994-01-14

1996-01-09

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

530350, C12N 916, C07K 1421

Patent

active

054828473

ABSTRACT:
The present invention relates to a DNA fragment containing a nucleotide sequence that encodes an amino acid sequence of esterase, said esterase asymmetrically hydrolyzing carboxylic acid esters represented by the formula (I); ##STR1## (wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 are alkyl, and n is 1 or 2) an esterase encoded by the DNA fragment, a recombinant plasmid containing the DNA fragment, a microorganism transformed with the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters.

REFERENCES:
patent: 4629701 (1986-12-01), Sakimae et al.
patent: 5273897 (1993-12-01), Sato et al.
Choi et al., 1990, Agric. Biol. Chem. 54(8):2039-2045.
Kamphuis et al., 1987, Proc. 4th Eur. Congr. Biotechnol., 4:331-348.
London et al., 1984, App. Environ. Microbiol., 47(5):993-997.

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