Essentially purified, thermostable and alkalophilic lipase from

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435832, C12N 920, C12N 1575

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active

050932560

ABSTRACT:
A thermostable lipase is produced by aerobically culturing a microorganism having the identifying characteristics of Bacillus sp. A30-1 ATCC No. 53841 on a nutrient medium under growth conditions until a recoverable lipolytic activity is detectable and thereafter isolating the thermostable lipase. The thermostable lipase has an optimum pH of 9.5, an isoelectric point of 5.15, a molecular weight of about 65,000 and retaining approximately 100% of its original activity upon being heated at 75.degree. C. for 30 minutes.

REFERENCES:
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patent: 4798793 (1989-01-01), Eigtved
Bozoglu et al, Isolation and Characterization . . . , J. Agric. Food Chem., 32, pp. 2-6, 1984.
Thermophilic Bacterial Isolates Producing Lipase, FEMS Microbiol. Lett., 48:339-343, 1987, Pauline Gowland, Martin Kernick and Trichur K. Sundaram.
A Thermostable Bacillus Lipase Res. Disclosure, 1987, 275:121.
Lipase Production by Bacillus Stearothermophilus S.203 in Shake Flasks, Zbl. Mikrobiol. 139, 61-68, 1984, M. S. Ammar and L. E. McDaniel.

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