Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex
Patent
1991-08-12
1994-12-06
Kim, Kay K. A.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Conjugate or complex
42419711, 4242571, 530395, 530404, 530405, 530406, 530411, A61K 39116, A61K 39385, C07K 1710
Patent
active
053708725
ABSTRACT:
The present invention relates to a method of producing an E. coli vaccine and to the vaccine produced thereby. The method involves purifying lipopolysaccharide from E. coli expressing complete O-polysaccharide sidechains;isolating the O-polysaccharide region of the lipopolysaccharide molecule by hydrolysis in dilute acetic acid and purifying it essentially free of lipid A; and covalently coupling lipid A-free O-polysaccharide via at least one hydroxyl or carboxyl group of the polysaccharide to a carrier protein. Polyvalent vaccines are prepared by combining two or more monovalent vaccines for different serotypes prepared according to the present invention. The present also relates to conjugates used in the vaccines. The conjugates of the present invention are the O-polysaccharide region of an E. coli lipoplysaccharide molecule covalently coupled to a carrier protein.
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Cryz Stanley J.
Furer Emil P.
Cordell Ruffin B.
Kile Bradford E.
Kim Kay K. A.
O'Brien Kevin M.
Swiss Serum and Vaccine Institute Berne
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