Escherichia coli (e.coli) test method

Details Escherichia coli (e.coli) test method Escherichia coli (e.coli) test method

1987-04-17

1990-05-08

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435 30, 435 34, 435 18, 435849, C12Q 110

Patent

active

049238043

ABSTRACT:
The present invention provides a method which is specific to determining and is capable of enumerating Escherichia coli (E. coli) in a sample water or sewage specimen. The method comprises adding to a cultured specimen a chromogenic reagent which when subjected to in situ E. coli .beta.-glucuronidase activity produces clearly defined vivid colorization of any individual colonies derived from E. coli cells in the test specimen.

REFERENCES:
patent: 4277561 (1981-07-01), Monget et al.
Anderson et al., Substrates for the Histochemical Localization of Some Glycosidases, 1961, pp. 236-238.
Pearson et al., "Histochemical .beta.-Glucuronidase Distribution in Mammalian Tissue as Detected by 5-Bromo-4-Chloroindol-3-yl-.beta.-D-Glucopyruroniside", 1967, vol. 17, No. 2, pp. 217-224 of the periodical entitled Laboratory Investigation.

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