Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Reexamination Certificate
2008-02-29
2010-10-26
Mondesi, Robert B (Department: 1645)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
C435S069100
Reexamination Certificate
active
07820411
ABSTRACT:
A method of producing botulinum toxin C-terminal receptor binding domain (HCR) is disclosed. The one embodiment, the method comprises the steps of (a) preparingE. colitransformed with an expression vector comprising DNA encoding HCR protein, (b) inducing expression of the HCR protein at a reduced temperature in a culture media, and (c) purifying the HCR protein via extraction, wherein the extraction comprises a clarification by centrifugation and a filtration, wherein the purified HCR protein is at least 10 mg/L of culture medium.
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Carstens et al. (Strategies Newsletter, 14:50-52, 2001).
Baldwin et al. (Prot. Exp. Pur., 37:187-195, Sep. 2004).
Lalli et al. (J. Cell Sci., 112:2715-2724, 1999).
Baldwin, M., et al., “The C-terminus of botulinum neurotoxin type A light chain contributes to solubility, catalysis and stability”, Protein Expr. Purif., 2004, 37:187-195.
Baldwin Michael
Barbieri Joseph T.
Bradshaw Marite
Johnson Eric A.
Pier Christina L.
Gangle Brian J
Mondesi Robert B
Quarles & Brady LLP
Wisconsin Alumni Research Foundation
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