Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1993-11-12
1996-12-10
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4351723, 435202, 4353201, 435836, 536 232, 536 237, 530350, 530825, C12N 120
Patent
active
055830390
ABSTRACT:
An Escherichia coli (ATCC 68319) containing a gene encoding a novel maltogenic amylase of Bacillus licheniformis (BLMA), wherein said gene has a length of about 3.5 kb; said gene expresses a gene product capable of hydrolyzing cyclodextrin, pullulan, as well as starch at an optimum temperature of about 50.degree. C. at a pH of about 7; said gene product has sugar transferase activity in the presence of glucose; and said Escherichia coli produces said gene product optimally when grown in Luria broth, for 24 hours, at 37.degree. C.
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Joyet, P. et al., "Cloning of a thermostable .alpha.-amylase gene from Bacillus licheniformis and its expression in Escherichia coli and Bacillus subtilis", FEMS Microbiology Letters 21 (1984) 353-358.
Kim, In-Cheol et al., "Catalytic Properties of the Cloned Amylase from Bacillus licheniformis", The Journal of Biological Chemistry, vol. 267, No. 31 Issue of Nov. 5, pp. 22108-22114 (1992).
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Cha Jae H.
Choi Yang D.
Jang So Y.
Kim In C.
Kim Jeoung R.
Doosan Technical Center
Dunn Michael L.
LeGuyader John L.
Sunhill Glucose Co., Ltd.
Sunkyong Industries Ltd.
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