Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1985-12-24
1989-11-07
Teskin, Robin
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435320, 435 91, 435255, 435256, 43525233, 536 27, 935 28, 935 37, 935 56, 935 69, 935 73, C12N 1500, C12N 500, C12P 1934
Patent
active
048792306
ABSTRACT:
The present invention relates to plasmids whose hosts can be Escherichia coli and some kinds of yeasts, namely, shuttle vectors, as well as to processes for producing said plasmids. There are provided in the present invention (1) plasmids containing an autonomously replicating sequence of Candida maltosa, Leu 2 gene derived from Saccharomyces cerevisiae and an ampicillin resistance gene and (2) plasmids further containing a tetracycline resistance gene as well as the genes described in (1).
The plasmids (shuttle vectors) of the present invention can be utilized as follows. A useful foreign gene is inserted into plasmids of the present invention; using the resulting new plasid, Escherichia coli is transformed and cultured in order to obtain the plasmid in a large amount; and using this plasmid, Saccharomyces cerevisiae or Candida maltosa as a host is allowed to produce useful substances such as hormones and enzymes on a large scale.
REFERENCES:
Takagi et al., J. Bact., vol. 167(2) pp. 551-555, Aug. 1986, "Construction of a Host-Vector System in Candida maltosa by Using an ARS Site Isolated from Its Genome".
Gillum et al., Mol Gen Genet, vol. 198, pp. 179-182, 1984, "Isolation of the Candida albicans Gene for orotidine-5'-phosphate decarboxylyse by complementation of S. cerevisiae ura3 and E coli pyrF Mutations".
Broach et al., Gene. vol. 8, pp. 121-133, 1979.
Ho et al., Chem. Abst., vol. 103(5) No. 32483j, 1984, "Development of a Cloning System for Candida Species".
Kawamura et al., Gene, vol. 24, pp. 157-162, 1983, "Cloning of a Leu Gene and an ARS Site of Candida maltosa".
Rosenbluh et al., Mol Gen Genet, vol. 200, pp. 500-502, 1985, "Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae".
Hsu et al., J. Bact., vol. 154(3) Jun. 1983, pp. 1033-1039, "Construction of a New Yeast Cloning Vector Containing Autonomous Replication Sequences from Candida utilis".
Morikawa Minoru
Shibuya Ichiro
Takagi Masamichi
Yano Keiji
Nikka Whisky Distilling Co., Ltd.
Teskin Robin
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