Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1985-06-20
1987-05-19
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
424 95, 424 99, 424101, 435 68, 4351722, 4351723, 435240, 435241, 435948, 514 6, 530380, 530395, 530399, 530416, 530808, 530809, 530834, 935109, A61K 3724, A61K 3522, A61K 3736, C07K 1514
Patent
active
046670165
ABSTRACT:
Chromatographic procedures are individually and jointly applied to the rapid and efficient isolation of biologically active proteins and especially glycoproteins such as recombinant erythropoietin present in the medium of growth of genetically transformed mammalian host cells. Illustratively, recombinant EPO is isolated from culture fluids by reverse phase chromatography employing a C.sub.4 or C.sub.6 column and elution with ethanol. Recombinant erythropoietin may also be purified by anion exchange chromatography employing, e.g., a DEAE resin, with preliminary selective elution of contaminant materials having a lower pKa than erythropoietin from the resin under conditions mitigating against acid activated protease degradation. Practiced serially, the two chromatographic procedures allow for high yields of biologically active recombinant erythropoietin from mammalian cell culture media.
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Lai Por-Hsiung
Strickland Thomas W.
Kirin-Amgen Inc.
Schain Howard E.
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