Erythropoietin conjugates

Details Erythropoietin conjugates Erythropoietin conjugates

2006-10-31

2006-10-31

Nashed, Nashaat T. (Department: 1656)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Conjugate or complex

C530S397000, C530S350000, C514S002600, C514S008100, C424S194100, C930S010000, C930S090000

Reexamination Certificate

active

07128913

ABSTRACT:
The present invention refers to conjugates of erythropoietin with poly(ethylene glycol) comprising an erythropoietin glycoprotein having an N-terminal α-amino group and having the in vivo biological activity of causing bone marrow cells to increase production of reticulocytes and red blood cells and selected from the group consisting of human erythropoietin and analogs thereof which have the sequence of human erythropoietin modified by the addition of from 1 to 6 glycosylation sites or a rearrangement of at least one glycosylation site; said glycoprotein being covalently linked to one poly(ethylene glycol) group of the formulain-line-formulae description="In-line Formulae" end="lead"?—CO—(CH2)x—(OCH2CH2)m—ORin-line-formulae description="In-line Formulae" end="tail"?wherein the —CO of the poly(ethylene glycol) group forms an amide bond with said N-terminal α-amino group; and wherein R is lower alkyl; x is 2 or 3; and m is from about 450 to about 1350.

REFERENCES:
patent: 5272071 (1993-12-01), Chappel
patent: 5547933 (1996-08-01), Lin
patent: 5621080 (1997-04-01), Lin
patent: 5641670 (1997-06-01), Treco et al.
patent: 5672662 (1997-09-01), Hedgepeth et al.
patent: 5733761 (1998-03-01), Treco et al.
patent: 5981214 (1999-11-01), Skoultchi
patent: 6340742 (2002-01-01), Burg et al.
patent: 6583272 (2003-06-01), Bailon
patent: 0 148 605 (1985-07-01), None
patent: 0 205 564 (1986-12-01), None
patent: 0 209 539 (1987-01-01), None
patent: 0 267 678 (1988-05-01), None
patent: 0 307 247 (1989-03-01), None
patent: 0 343 635 (1989-11-01), None
patent: 0 481 791 (1992-04-01), None
patent: 0 513 738 (1992-11-01), None
patent: 0 605 963 (1994-07-01), None
patent: 0 640 619 (1995-03-01), None
patent: 0 248 656 (1997-12-01), None
patent: 1 064 951 (2001-01-01), None
patent: WO 88/00967 (1988-02-01), None
patent: WO 90/11354 (1990-10-01), None
patent: WO 94/01451 (1994-01-01), None
patent: WO 96/11953 (1996-04-01), None
patent: WO 96/35718 (1996-11-01), None
patent: WO 96/41813 (1996-12-01), None
patent: WO 97 03106 (1997-01-01), None
patent: WO 97/09996 (1997-03-01), None
patent: WO 97/40850 (1997-11-01), None
patent: WO 98 32466 (1998-07-01), None
patent: WO 98/05363 (1998-12-01), None
patent: WO 98/58660 (1998-12-01), None
patent: 99/07735 (1999-02-01), None
patent: WO 99/07401 (1999-02-01), None
patent: WO 00 32772 (2000-06-01), None
patent: WO 0042175 (2000-07-01), None
patent: WO 01 02017 (2001-01-01), None
patent: WO 01 87329 (2001-11-01), None
US 5,733,746, 03/1998, Treco et al. (withdrawn)
Bill et al. Expression and mutagenesis of recombinant human and murine erythropoietins inEscherichia coli. (1995) Biochim. Biophys. Acta. 1261: 35-43.
Wen et al. Erythropoietin Structure-Function Relationships: High Degree of Sequence Homology Among Mammals. (1993) Blood 82(5): 1507-1516.
Bavister, B., Exp. Zoology vol. 271 (1981) pp. 45-51.
Dalbey, R.E. et al., Protein Sci. vol. 6, pp. 1129-1138.
Danna, R. P., Rudnick, S. A., Abels, R. I., In: MB, Garnick, ed. Erythropoietin in Clinical Applications-An International Perspective. New York, NY: Marcel Dekker, 1990, pp. 301-324.
Egrie, J. C., Strickland, T.W., Lane, J. et al. (1986) Immunobiol. vol. 72 pp. 213-224.
Egrie, J. C., Eschbach, J. W., McGuire, T., Adamson, J.W. (1988) Kidney Intl. vol. 33 pp. 262-263.
Erslev, A. J. (1953) Blood vol. 8, pp. 349-357.
Eschbach, J. W., Egri J. C. Downing, M. R. et al., (1987) NEJM vol. 316 pp. 73-78.
Eschbach, J. W., Abdulhadi, M. H. Browne, J. K. et al., (1989) Ann. Intern. Med. vol. 111 pp. 992-1000.
Gaertner, H. F., Offord, R.E., Biconjugates Chem. vol. 7(1) pp. 38-44 (1996).
Huang, Proc. Natl. Acad. Sci. USA (1984) pp. 2708-2712.
Jacobson, L. O. Boldwasser, E., Freid, W. and Plzak, LF (1957) Nature vol. 179 pp. 633-634.
Kawamoto, T. et al, Analytical Biochem. vol. 130 (1983) pp. 445-453.
Kowar, J. and Franek, R., Methods in Enzymology vol. 121 (1986) pp. 277-292.
Krantz, B. S. (1991) Blood vol. 77 pp. 419-434.
Lai, P. H., J. Biol. Chem. vol. 261 (1986) pp. 3116-3121.
Lim, V.S., Degowin, R. L., Zavala, D. et al. (1989) Ann. Intern. Med. vol. 110 pp. 108-114.
Reissmann, K. R. (1950) Blood vol. 5, pp. 372-380.
Sasaki, H., Bothner, B., Dell, A., and Fukuda, M., (1987), J. Biol, Chem., vol. 262 pp. 12059-12076.
Sato, H., Yamamoto, K., Hayashi, E., Takahara, Y., Biconjugates Chem. vol. 11(4) pp. 502-509, 2000.
Zhao, Z. G., Im, J.S. Clarke, D. F. Biconjugates Chem. vol. 10, pp. 424-430 (1999).

Affiliated with

Also associated with

Rating

Erythropoietin conjugates does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Erythropoietin conjugates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Erythropoietin conjugates will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3671726