Erythrocyte agglutination assay

Details Erythrocyte agglutination assay Erythrocyte agglutination assay

1988-01-13

1990-01-16

Richman, Barry S.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

436501, 436519, 422 61, 530387, G01N 33541

Patent

active

048943479

ABSTRACT:
In an erythrocyte agglutination assay, the agglutination reagent comprises at least one erythrocyte binding molecule coupled to at least one specific analyte binding molecule wherein the erythrocyte binding molecule does not cause agglutination when incubated with erythrocytes in the absence of analyte. Preferably, the erythrocytes are endogenous to the blood sample to be tested, that is, a whole blood sample is assayed. Mixtures of conjugates and conjugates of analyte analogues with erythrocyte binding molecules may also be used as agglutination reagents. The reagents and their use in direct or indirect assays is disclosed.

REFERENCES:
patent: 4433059 (1984-02-01), Chang et al.
patent: 4470925 (1984-09-01), Auditore-Hargreaves
patent: 4529712 (1985-07-01), Jou et al.
patent: 4578360 (1986-03-01), Smith
patent: 4594327 (1986-06-01), Zuk
patent: 4829011 (1989-05-01), Gibbons

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