Epoxide hydrolases of aspergillus origin

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S123000, C435S158000, C435S252300, C435S326000, C536S023200

Reexamination Certificate

active

07060477

ABSTRACT:
The invention concerns proteins of fungal origin having an epoxide hydrolase activity, such as those obtained in essentially pure form by extraction from fungi cells, or by culturing in host cells transformed by a nucleotide sequence coding for said fungal proteins. The invention also concerns the uses thereof, in particular for implementing methods for preparing enantiopure epoxides and/or diols.

REFERENCES:
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Morisseau, C., er all. (1999) Eur. J. Biochem. 263, 386-395.
Archelas et al, “Epoxide . . . Chemicals”, Trends in Biotechnology, GB, Elsevier Publications, Cambridge, vol. 16, No. 3, Mar. 1, 1998 pp. 108-116.
Moassou et al, “Microgiological Transformations 40. Use . . . Epoxides”, Tetrahedron, NL, Elsevier Science Publishers, Amsterdam, vol. 54, No. 8, Feb. 19, 1998 pp. 1563-1572.
Cleij et al, “Microbiological . . . Enhancement”, Tetrahedron: Asymmetry, NL, Elsevier Science Publishers, Amsterdam, vol. 09, No. 11, Jun. 5, 1998, pp. 1839-1842.
Nellaiah et al, “Enantioselective . . .Aspergillus niger”, Biotechnology and Bioengineering, vol. 49, 1996, pp. 70-77, XP002130510.
Kupfer et al, “An . . . Database”, Feb. 2, 1998, XP002130511.
Arand et al, “Cloning . . . Hydrolase”, Biochemical Journal, vol. 344, Nov. 15, 1999, pp. 273-280, XP000938551.

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