Epitope mapping of the c33 region of HCV

Details Epitope mapping of the c33 region of HCV Epitope mapping of the c33 region of HCV

1995-03-28

1998-05-05

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 435 792, 530350, C12Q 170, G01N 3353, G01N 33551, C07K 1418

Patent

active

057472408

ABSTRACT:
An assay for detection of serum antibodies having specificity to hepatitis C viral antigens utilizes the carboxy-terminal 102 amino acids of the c33c region of nonstructural gene 3 of HCV containing substantially all the epitopes of the gene 3 protein. In the preferred embodiment the recombinant 102 amino acid peptide is coated onto a solid support, and incubated in the presence of serum obtained from a patient infected with HCV. The presence of antibodies bound to the antigen coated support is detected in a sandwich format utilizing a second reporter-conjugated antibody.

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