Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1989-06-21
1995-05-30
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352401, 4351721, 4351723, 935 71, 935 70, 935 66, C12Q 102, C12N 506, C12N 1506
Patent
active
054200334
ABSTRACT:
An airway epithelial cell line (CF/T43) was developed by infecting cultured cystic fibrosis (CF) airway epithelial cells with the pZIPneoSV(X)1/SV40T retrovirus and selecting for Genetian (G418) resistance and ion transport properties. The distinctive chloride secretory phenotype of CF/T43 [an apical membrane chloride permeability (P.sub.cl -) activated by calcium-mediated, but not by adenosine 3',5'-monophosphate (cAMP)-dependent agonists] was not perturbed by SV40T-induced cell transformation. Epithelial cell lines generated from CF cells with the SV40T gene can be used to test candidate CF genes, to evaluate the molecular mechanisms responsible for the CF phenotype and to test putative therapeutic CF drugs.
REFERENCES:
patent: 5100647 (1992-03-01), Agus et al.
Yankaskas et al, Am. Rev. Respir. Dis., vol. 143, Abstract A139, 1991.
Scholte, B. J., et al., Experimental Cell Research, vol. 182 (Jun. 1989) 559-571.
Gruenert, D. C., et al., Proc. Natl. Acad. Sci. USA, vol. 85 (Aug. 1988) 5951-5955.
Jetten, A. M., et al., Science, vol. 244, (23 Jun. 1989) 1472-1475.
Lu, L., et al., Biological Abstracts, vol. 89, No. 4, abstract No. 37371 (1990).
Buchanan, J. A., et al., Journal of Cell Science, vol. 95 (1990) 109-123.
P. L. Zeitlin, et al., A Cystic Fibrosis Bronchial Epithelial Cell Line: Immortalization by Adeno-12SV40 Infection. vol. 4. pp. 331-319, 1991.
Douglas M. Jefferson, et al., Expression of Normal and Cystic Fibrosis Phenotypes by Continuous Airway Epithelial Cell Lines. Am. J. Physiol. 259: L496-L505 (1990).
Scheiner Toni R.
The United States of America as represented by the Secretary of
LandOfFree
Epithelial cell line expressing a cystic fibrosis phenotype does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Epithelial cell line expressing a cystic fibrosis phenotype, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Epithelial cell line expressing a cystic fibrosis phenotype will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-360950