Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1991-03-21
1994-08-02
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352402, 435240243, 623 15, C12N 500, A61F 210
Patent
active
053345274
ABSTRACT:
The present invention involves a readily produced skin autograft or silograft composite suitable for the supplementation or replacement of injured skin. A sheet of collagen-coated pliable material such as synthetic surgical dressing is used as a foundation for the skin autograft composite. The synthetic surgical dressing, if not precoated with collagen, may be placed in a container and coated with collagen. Epidermal cells, preferably obtained from a prospective recipient of the skin autograft composite being produced, are cultured in an appropriate medium substantially preventing cell differentiation to form epidermal cells on the surface of a collagen-coated container. When substantially confluent, the epidermal cells are enzymatically detached from the culture vessel and layered upon a collagen coated desiccated surgical dressing infused with low calcium culture medium. In actual use, the skin autograft composite is inverted, placed on a recipient, and allowed to remain in position until epidermal cells attach to the recipient and facilitate the formation of a skin-like covering.
REFERENCES:
patent: 3883393 (1975-05-01), Knazek et al.
patent: 4060081 (1977-11-01), Yannas et al.
patent: 4087327 (1978-05-01), Feder et al.
patent: 4220725 (1980-09-01), Knazek et al.
patent: 4254226 (1981-03-01), Eisinger et al.
patent: 4296205 (1981-10-01), Verma
patent: 4299819 (1981-11-01), Eisinger
patent: 4308351 (1981-12-01), Leighton et al.
patent: 4399123 (1983-08-01), Oliver et al.
patent: 4485096 (1984-11-01), Bell
patent: 4495288 (1985-01-01), Jarvis, Jr. et al.
patent: 4504547 (1985-03-01), Horodniceau et al.
patent: 4533635 (1985-08-01), Guedon born Saglier et al.
patent: 4539716 (1985-09-01), Bell
patent: 4769317 (1988-09-01), Hefton
patent: 5000963 (1991-03-01), Hefton
patent: 5015584 (1991-05-01), Brysk
Kitano and Endo, Biochemistry of Cutaneous Epidermal Differentiation, Ed. by Seiji, et al., University Park Press, pp. 319-335 (1977).
Pittelkow, Mayo Clin. Proc, 61:771-777 (1986).
The Lancet, "Culture Crafts of Keratinocytes: A Growth Industry?" p. 783, Apr. 5, 1986.
Thivolet, et al, Transplantation, 42:27.
Dermatology Perspectives, Ed. by Gahagan, "Synthetic dressings speed healing and reduce pain" pp. 2-3 (1986).
Eaglstein, J. of Am. Acad. Dermatol., 12:434-440 (1985).
Eisinger, et al., Surgery, pp. 287-293 (1980).
Gallico, et al., The New England Journal of Medicine, 311:448-51.
Green, et al., Proc. Natl., Acad. Sci, U.S.A., 76:5665-5668 (1979).
Boyce, et al., J. Tissue Culture Methods, vol. 9, No. 2, 1985, pp. 83-93.
Biomaterials, vol. 6, No. 4, Nov. 1985, Butterworth & Co. Ltd., K. J. Quinn et al.
La Recherche, vol. 14, No. 141, Feb. 1983, (Paris, FR), C. Tutin.
WO, A 83/01385 (Massachusetts Institute of Technology), Apr. 28, 1983.
WO, A, 83/01384 (Massachusetts Institute of Technology), Apr. 28, 1983.
International Search Report.
Rheinwald and Green, Cell, 6:311-344 (1975).
Board of Regents , The University of Texas System
Dadio Susan M.
Robinson Douglas W.
LandOfFree
Epidermal graft system does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Epidermal graft system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Epidermal graft system will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-64559