Eph-related tyrosine kinases, nucleotide sequences and methods o

Details Eph-related tyrosine kinases, nucleotide sequences and methods o Eph-related tyrosine kinases, nucleotide sequences and methods o

1993-12-03

1995-10-10

Furman, Keith C.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 435194, 536 232, 536 235, C12N 912, C12N 1554

Patent

active

054570484

ABSTRACT:
The invention is directed to substantially purified Eph-related protein tyrosine kinases, or functional fragments thereof, having about 23 to 66 percent amino acid sequence identity in their carboxyl terminal variable regions compared to known members of the Eph subclass of tyrosine kinases. Nucleic acids encoding such Eph-related protein tyrosine kinases, vectors and host cells are also provided. The invention is also directed to a method of diagnosing cancer and determining cancer prognosis. The method includes removing a tissue or cell sample from a subject suspected of having cancer and determining the level of Eph-related protein tyrosine kinase in the sample, wherein a change in the level or activity of a Eph-related protein tyrosine kinase compared to a normal sample indicates the presence of a cancer or indicates the level of malignancy of a cancer.

REFERENCES:
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Roux et al (1980) BioTechniques 8(1) 48-57.
Sambrook et al. in "Molecular Cloning: A Laboratory Manual" 2nd Ed. CHS Press, (1989) pp. 11-2-11.57.
Lhotak, Vladmir, et al. "Characterization of Elk, a Brain-Specific Receptor Tyrosine Kinase." Mole. Cell. Biol. 11(5):2496-2502 (1991).
Gilardi-Hebenstreit, Pascale et al. "An Eph-Related Receptor Protein Tyrosine Kinase Gene Segmentally Expressed in the Developing Mouse Hindbrain." Oncogene 7:2499-2506 (1992).
Nieto, M. Angela et al. "A Receptor Protein Tyrosine Kinase Implicated in the Segmental Patterning of the Hindbrain and Mesoderm." Development 116:1137-1150 (1992).
Letwin, Kenneth et al. "Novel Protein-Tyrosine Kinase cDNAS Related to fps/fes and eph Cloned Using Anti-Phosphotyrosine Antibody." Oncogene 3:621-627 (1988).
Maisonpierre, Peter C. et al. "Ehk-1 and Ehk-2: Two Novel Members of the Eph Receptor-like Tyrosine Kinase Family with Distinctive Structures and Neuronal Expression." Oncogene 8:3277-3288 (1993).
Bohme, Beatrix, et al., "PCR Mediated Detection of a New Human Receptor-Tyrosine-Kinase, HEK 2." Oncogene 8:2857-2862 (1993).

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