Enzymic method of detecting analytes and novel substrates theref

Details Enzymic method of detecting analytes and novel substrates theref Enzymic method of detecting analytes and novel substrates theref

1988-03-15

1991-10-15

Saunders, David A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 435 791, 435 18, 435 19, 435810, 435966, 435975, 436501, C12Q 168, G01N 33535

Patent

active

050574124

ABSTRACT:
A small enzymically inactive peptide fragment of an enzyme (e.g. ribonuclease S-peptide) is used as the label and conjugated with the complementary fragment (S-protein) to form an enzyme which catalyses a primary reaction whose product is, or leads to, an essential coenzyme or prosthetic group for a second enzyme which catalyses a secondary reaction leading to a detectable result indicating the presence of analyte. Also disclosed are novel synthetic substrates for the primary reaction. Substrates for ribonuclease S conjugate enzyme are of the formula R-X where R is pyrimidine 3'-phosphate moiety and X is a leaving group linked to R through the 3'-phosphate group and leads to said coenzyme or prosthetic group, e.g. via riboflavin, thiamine, pyridoxal, pyridoxine or pyridoxine phosphate.

REFERENCES:
patent: 4463090 (1984-07-01), Harris
patent: 4598042 (1986-07-01), Self
patent: 4745054 (1988-05-01), Rabin et al.

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