Enzymic assay for acetate ion

Details Enzymic assay for acetate ion Enzymic assay for acetate ion

1976-05-10

1977-07-12

Tanenholtz, Alvin E.

Textiles: fiber preparation

195 99, G01N 3114, G01N 3300

Patent

active

040352390

ABSTRACT:
An improved enzymic assay and assay composition for acetate ion in a biological fluid (e.g., blood serum or tissue extract) or a solution of fruit juice origin (e.g., wine). The acetate ion is reacted with adenosine triphosphate in the presence of enzymic quantities of phosphotransacetylase to form adenosine diphosphate. The above procedure is accelerated by the presence of Coenzyme A and phosphotransacetylase enzyme during the formation of adenosine diphosphate. Thereafter, the quantity of adenosine diphosphate may be assayed by known enzymic techniques. The procedure may be employed for analysis of acetate esters (e.g., ethyl acetate) by hydrolysis to acetate ion in the presence of esterase.

REFERENCES:
kuo et al. "A Specific Micromethod for Determination of Acetyl Residues in Proteins" Analytical Biochem. 55, 1-8 (1973).
Bergmeyer et al. "Method of Enzymatic Analysis" Verlag Chemie Weinheim, AP, Inc. N.Y., San Francisco, London (1974) pp. 1520-1528.

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