Enzymes with lipase/acyltransferase activity, nucleic acids...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S198000, C536S023200

Reexamination Certificate

active

10681636

ABSTRACT:
Polypeptides with lipase/acyltransferase activity are described with an amino acid sequence which has identity to the sequence reported in SEQ ID NO 2 of at least 49%, also polypeptides which display this activity, as well as nucleic acids (genes) which code for these polypeptides, vectors which contain nucleic acids which code for these polypeptides, transformed microorganisms which contain these nucleic acids, processes for production of these polypeptides and the application of nucleic acids for discovering new lipase/acyltransferases and the use of these lipase/acyltransferases as catalysts in chemical and biochemical processes.

REFERENCES:
patent: 197 53 789 (1999-06-01), None
Sequence search alignment between SEQ ID No. 2 and accession No. Q8NIN8.
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Vaysse, L., et al., “Fatty hydroxamic acid biosynthesis in aqeous medium in the presence of the lipase-acyltransferase fromCandida parapsilosis”, J. of Biotechnology, 53:41-46 (1997).
EMBL database, entry name: Z9P8W1, Gene name: LIP4, accessed Dec. 13, 2001.
EMBL database, gene: LIP1, accessed Dec. 13, 2001.
Fu, Y., et al., “Cloning and characterization of a gene (LIP1) which encodes a lipase from the pathogenic yeastCandida albicans”, Microbiology 143:331-340 (1997).
EMBL database, entry name: O94015, gene name: CA35A5.09, accessed Dec. 13, 2001.
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Abstract Briand, D., et al., “Functioning and regioselectivity of the lipase ofCandida-parapsilosis(Ashford) langeron and talice in aqueous-medium—New interpretation of regioselectivity taking acyl migration into account”, European Journal of Biochemistry, 228:169-175 (1995).
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