Enzymes involved in triterpene synthesis

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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C800S278000, C800S285000, C800S306000, C800S312000, C435S419000, C435S320100, C536S023100, C536S023200, C536S023600

Reexamination Certificate

active

07135624

ABSTRACT:
This invention relates to an isolated nucleic acid fragment encoding an oxidosqualene cyclase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to a suitable regulatory sequence.

REFERENCES:
patent: WO 95/30009 (1995-11-01), None
patent: WO 01/46391 (2001-06-01), None
GenBank Accessions AB014057 and AB009030.
Kushiro T. et al. Eur. J. of Biochemistry, 1998; vol. 256, No. 1, pp. 238-244.
Wu T. et al. Biochemistry, 2002; vol. 41 pp. 8238-8244.
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National Center for Biotechnology Information General Identifier No. 3721856, Oct. 9, 1998, Kushiro, T. et. al., Molecular Cloning of Oxidosqualene Cyclase CDNA From Panax Ginseng: The Isogene that Encodes Beta-Amyrin Synthase.
National Center for Biotechnology Information General Identifier No. 3688600, Oct. 3, 1998, Kushiro, T. et. al., Beta-Amyrin Synthase-Cloning of Oxidosqualene Cyclase That Catalyzes the Formation of the Most Popular Triterpene Among Higher Plants.
National Center for Biotechnology Information General Identifier No. 3779033, Oct. 22, 1998, Rounsley, S.D. et. al.,Arabidopsis thalianaChromosome II Bac T4E14 Genomic Sequence.
National Center for Biotechnology Information General Identifier No. 5606831, Jul. 27, 1999, Shoemaker, R. et. al., Public Soybean EST Project.
Accession No. AB009030, Oct. 5, 1998, Kushiro T. et. al, Cloning of Oxidosqualene Cyclase That Catalyzes the Formation of the Most Popular Triterpene Among Higher Plants.
Tetsuo Kushiro et. al., Eur. J. Biochem., vol. 256:238-244, 1998, Cloning of Oxidosqualene Cyclase That Catalyzes the Formation of the Most Popular Triterpene Among Higher Plants.
Masaaki Shibuya et. al., Eur. J. Biochem., vol. 266:302-307, 1999, Two Branches of the Lupeol Synthases Gene in the Molecular Evolution of Plant Oxidosqualene Cyclases.
Hiroaki Hayashi et. al., Biol. Pharm. Bull., vol. 23-231-234, 2000, Molecular Cloning and Characterization of a Cdna for Glycyrrhiza Glabra Cycloartenol Synthase.
Database Accession No. 94:267952, 1994, C. Tsukamoto et al., ACS Symposium Series—General Review—Journal, Genetic Improvement of Saponin Components in Soybean.
Chigen Tsukamoto et. al., Phytochemistry, vol. 34:1351-1356, 1993, Genetic and Chemical Polymorphisms of Saponins in Soybean Seed.
Akio Kikuchi et. al., Breeding Science, vol. 49:167-171, 1999, Inheritance and Characterization of a Null Allele for Group a Acetyl Saponins Found in a Mutant Soybean (Glycine Max(L) Merrill).
Chigen Tsukamoto et. al., J. Agric. Food Chem., vol. 43:1184-1192, 1995, Factors Affecting Isoflavone Content in Soybeans Seeds: Changes in Isoflavones, Saponins, and Composition of Fatty Acids at Different Temperatures During Seed Development.
Yumiko Yoshiki et. al., Biosci. Biotechnol. Biochem., vol. 62:2291-2299, 1998, Relationship Between Chemical Structures and Biological Activities of Triterpenoid Saponins From Soybean.
Edmund W. Lusas et. al., Journal of Nutrition, vol. 125:573S-580S, 1995, Soy Protein Products: Processing and Use.
Thomas J. Bach et. al., Progress in Lipid Research, vol. 36:197-226, 1997, Cloning of CDNAS or Genes Encoding Enzymes of Sterol Biosynthesis From Plants and Other Eukaryotes: Heterologous Expression and Complementation Analysis of Mutants for Functional Characterization.
Tung-Kung Wu et al., Conversion of a Plant Oxidosqualene-Cycloartenol Synthase to an Oxidosqualene-Lanosterol Cyclase by Random Mutagenesis, Biochemistry, 2002, vol. 41:8238-8244.
Ikuro Abe et al., Active Site Mapping of Affinity-labeled Rat Oxidosqualene Cyclase, The Journal of Biological Chemistry, Jan. 14, 1994, vol. 269, No. 2:802-804.
Karl Poralla et al., A specific amino acid repeat in squalene and oxidosqualene cyclases, Elsevier Science Ltd., Apr. 1994, 157-158.
EMBL Sequence Database, Accession No. 082140, Nov. 1, 1998, T. Kushiro et al., Beta-Amyrin Synthase—Cloning of Oxidosqualene Cyclase That Catalyzes the Formation of the Most Popular Triterpene Among Higher Plants, XP-002170235.
EMBL Sequence Database, Accession No. A1900929, Jul. 28, 1999, R. Shoemaker et al. Public Soybean EST Project, XP-002170236.
K. Papadopoulou et al., Compromised Disease Resistance in Saponin-Deficient Plants, PNAS, vol. 96, No. 22:12923-12928, Oct. 26, 1999.
Song You et al., Molecular Cloning and Sequencing of an Allium Macrostemon cDNA Probably Encoding Oxidosqualene Cyclase, Plant Biotechnology, 16 (4):311-214, 1999.
EMBL Sequence Database, Accession No. Q9SSZ2, May 1, 2000, S. You et al., Molecular Cloning and Sequencing of an Allium Macrostemon CDNA Probably Encoding Oxidosqualene Cyclase, XP-002170238.
EMBL Sequence Database, Accession No. AF169966, Oct. 21, 1999, L. B. Darr et al., A Rice CDNA Similar to Cycloartenol Synthase, XP-002170239.

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