Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1986-02-24
1989-02-28
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435 14, 435 25, 435 26, 435 28, 435177, 435180, 435182, 435288, C12N 1112, C12N 1108, C12Q 132, C12Q 128
Patent
active
048085294
ABSTRACT:
Proteins such as enzymes are attached directly to a polyamide or cellulose hydrate carrier. Attachment can be carried out by impregnation of the carrier with a protein solution. The carrier can be in the form of a membrane, and the polyamide can be nylon. Resulting carrier-bound proteins find utility as biocatalyst, as test strips or as chromatography materials. In a preferred embodiment, a polyamide or cellulose hydrate membrane carrier is impregnated with an enzyme selected from peroxidase, glucose oxidase, diaphorase and glucose dehydrogenase and then dried, coated with a luminol solution and again dried. The resultant membrane can be used for glucose determination by measuring chemiluminescence produced when the membrane contacts a glucose solution.
REFERENCES:
patent: 3705084 (1972-12-01), Reynolds
patent: 4231754 (1980-11-01), Vogelhut
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, pp. 79-82.
Olson et al., Immobilized Enzymes in Food and Microbiol Processes, Plenum Press, N.Y., 1974, pp. 63-69.
Doppelfeld Peter
Oosta Gary
Riebel Alexander
Schranz Karl-Wilhelm
Coe Roger N.
Miles Inc.
Naff David M.
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