Enzymes immobilized on latex polymer particles for use with an a

Details Enzymes immobilized on latex polymer particles for use with an a Enzymes immobilized on latex polymer particles for use with an a

1988-10-26

1991-01-08

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

204403, 435 18, 435 25, 435180, 435288, 435817, C12Q 100, C12Q 126, C12N 1108, C12M 140

Patent

active

049835106

ABSTRACT:
Enzymes such as L-methionine gamma lyase, L-lysine decarboxylase, L-aspartase and L-tryptophanase are immobilized by contacting with a latex of polymer particles having a negative surface charge such that the electrophoretic mobility of the latex has a negative value of -2.0 10.sup.-8 m.sup.2 V.sup.-1 sec.sup.-1 or below when measured at pH 7 in 0.01M KNO.sub.3 and a polymer solids concentration of 30 mg/l. The polymer particles also have a hydrophobic surface such that the contact angle of a 1 microliter droplet of distilled water with a plane horizontal surface formed by drying and compacting the latex particles is 70.degree. or more. The resultant-enzyme/polymer particles can be used for assaying amino acids corresponding to the specific enzyme immobilized in aqueous solutions using an electrosensor comprising a pH-sensitive electrode located in a sensing zone or chamber containing the enzyme/polymer particles as an aqueous dispersion or in water-dispersible form.

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