Enzymes having dehalogenase activity and methods of use thereof

Details Enzymes having dehalogenase activity and methods of use thereof Enzymes having dehalogenase activity and methods of use thereof

2006-07-18

2006-07-18

Fredman, Jeffrey (Department: 1637)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S024300, C536S024310, C536S024330, C435S006120, C435S091200, C435S320100

Reexamination Certificate

active

07078504

ABSTRACT:
The invention relates to haloalkane dehalogenases and to polynucleotides encoding alkane dehalogenases. In addition methods of designing new dehalogenases and of use thereof are also provided. The dehalogenases have increased activity and at increased pH and temperature.

REFERENCES:
patent: 6632937 (2003-10-01), Swanson
patent: WO 98/36080 (1998-08-01), None
patent: WO-00/58517 (2000-10-01), None
patent: WO-02/22810 (2002-03-01), None
Matthews et al, Anal. Biochem. (1988) 169:1-25.
Krooshof, et al., “Repositioning the Catalytic Triad Aspartic Acid of Haloalkane Dehalogenase: Effects on Stability, Kinetics, and Structure”,Biochemistry, vol. 36, No. 31, pp. 9571-9580, 1997.
Rozeboom, et al., “Crystallization of Haloalkane Dehalogenase fromXanthobacter autotrophicus GJ10”, Journal of Molecular Biology, vol. 200, No. 3, pp. 611-612, 1988.
Keuning, et al., “Purification and Characterization of Hydrolytic Haloalkane Dehalogenase fromXanthobacter autotropicus GJ10”, Journal of Bacteriology, vol. 163, No. 2, pp. 635-639, 1985.
Schanstra, et al., “Specificity and Kinetics of Haloalkane Dehalogenase”,Journal of Biological Chemistry, vol. 271, No. 25, pp. 14747-14763, 1996.
Verschueren, et al., “Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase”,Nature, vol. 363, No. 6431, pp. 693-698, 1993.
Affholter et al.; Rhodococcus haloalkane dehalogenase gene in vector pEXPROK; Dec 7, 1998 (first entry); Database GenBank (Geneseq); Accession No. AAV47592.
Poelarends et al.; Mycobacterium sp. dhaAf gene, strins GP1; Apr. 9, 1999; Database GenBank (GenEmbl); Accession No. MSP012627.
Poelarends et al.;Pseudomonas pavonaceaehaloalkane dehalogenase gene region; Apr. 7, 2000; Database GenBank (GenEmbl); accession No. PPA250371.
International Search Report mailed on Jul. 27, 2004, for PCT patent application No. PCT/US01/45337 filed on Nov. 30, 2001.
Supplementary Partial European Search Report for Application No. EP 01 27 3846, mailed on Jul. 20, 2005, 5 pages.
Supplementary Partial European Search Report for Application No. EP 01 27 3846, mailed on Sep. 23, 2005, 8 pages.
Damborsky et al., Protein Engineering (1998) 11:901-907.
Holloway et al., Biotechnology and Bioengineering (1998) 59:520-523.
Kulakova et al., Microbiology (1997) 143 (Part 1):109-115.
Newman et al., Biochemistry (1999) 38:16105-16114.

Affiliated with

Also associated with

Rating

Enzymes having dehalogenase activity and methods of use thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymes having dehalogenase activity and methods of use thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymes having dehalogenase activity and methods of use thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3546283