Enzymes for the production of 2-keto-L-gulonic acid

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435189, 435 691, 536 232, 930240, C12N 904

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active

053765443

ABSTRACT:
Mutants of 2,5-diketo-D-gluconic acid reductase A, an enzyme used to produce 2-keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C) are prepared by site-directed mutagenesis. These mutants have increased catalytic activity, increased expression levels, and/or enhanced temperature stability.

REFERENCES:
patent: 5008193 (1991-04-01), Anderson et al.
Anderson et al. 1985, Science 230, 144-149.
Rastetter, W. H. 1983, Trends Biotechnol. 1, 80-84.
Sonoyama, et al. 1987, J. Ferment. Technol. 65,311-317.
Imanaka et al. 1986, Nature 324, 695-697.
Grindley et al. 1988, Appl. Environ. Microbiol. 54, 1770-1775.

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